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Lab Dept:
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Serology
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Test Name:
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ANTI–GBM ANTIBODY
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General Information
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Lab Order Codes:
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GBM
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Synonyms:
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Glomerular Basement Membrane Antibody - Ig, Serum; Anti-GMB; Antiglomerular Basement Membrane Antibody – IgG; Basement Membrane Antibody; Basement Membrane, Glomerular Antibody; GBM (Glomerular Basement Membrane Antibody); GBM Antibody; Glomerular Anti-Basement Membrane; Goodpasture’s Syndrome, kidney or lung biopsy
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CPT Codes:
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83520 – Immunoassay, analyte, quantitative; not otherwise specified
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Test Includes:
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Glomerular Basement Membrane IgG antibody reported as negative or positive.
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Logistics
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Test Indications:
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Useful for evaluation of patients with rapid onset renal failure or pulmonary hemorrhage or as an aid in the diagnosis of Goodpasture’s syndrome.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test: GBM/8106)
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 3 days, test is set up Sunday - Friday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL (Minimum: 1.1 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.35 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen and remove serum aliquot. Store and send specimen at refrigerated temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Gross hemolysis; warm specimens; gross lipemia; mislabeled specimens or unlabeled specimens
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Interpretive
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Reference Range:
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All Ages:
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<1.0 U
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Negative
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≥1.0 U
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Positive
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Interpretation: Positive results are consistent with Goodpasture's syndrome. GBM antibodies detected by immunoassay have been reported to be highly specific for Goodpasture’s syndrome. The sensitivity of this test approaches 87% in untreated patients with systemic disease.
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Critical Values:
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N/A
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Limitations:
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A positive test for anti-GBM cannot be relied upon exclusively to establish the diagnosis of a disease mediated by GBM antibodies. Weakly positive test results may occur in other immune-mediated diseases, and renal or lung biopsy is often required to establish the diagnosis.
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Methodology:
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Multiplex Flow Immunoassay
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References:
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Mayo Medical Laboratories Web Page May 2013
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Updates:
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3/24/2006: Reference range change previously listed as ≤5.0 EU/mL (negative); 5.1 – 20.0 EU/mL (borderline); 20.1 – 400.0 EU/mL (positive)
11/18/2008: Reference range change at MML.
4/19/2011: Method change, previously listed as Enzyme Linked Immunosorbent Assay (ELISA) Enzyme Immunoassay (EIA).
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