Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

ANTI–GBM ANTIBODY

General Information

Lab Order Codes:

GBM

Synonyms:

Glomerular Basement Membrane Antibody - Ig, Serum; Anti-GMB; Antiglomerular Basement Membrane Antibody – IgG; Basement Membrane Antibody; Basement Membrane, Glomerular Antibody; GBM (Glomerular Basement Membrane Antibody); GBM Antibody; Glomerular Anti-Basement Membrane; Goodpasture’s Syndrome, kidney or lung biopsy

CPT Codes:

83520 – Immunoassay, analyte, quantitative; not otherwise specified

Test Includes:

Glomerular Basement Membrane IgG antibody reported as negative or positive.

Logistics

Test Indications:

Useful for evaluation of patients with rapid onset renal failure or pulmonary hemorrhage or as an aid in the diagnosis of Goodpasture’s syndrome.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (Test: GBM/8106)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days, test is set up Sunday - Friday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL (Minimum: 1.1 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.35 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen and remove serum aliquot. Store and send specimen at refrigerated temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Gross hemolysis; warm specimens; gross lipemia; mislabeled specimens or unlabeled specimens

Interpretive

Reference Range:

All Ages:

<1.0 U

Negative

≥1.0 U

Positive

Interpretation: Positive results are consistent with Goodpasture's syndrome. GBM antibodies detected by immunoassay have been reported to be highly specific for Goodpasture’s syndrome. The sensitivity of this test approaches 87% in untreated patients with systemic disease.

Critical Values:

N/A

Limitations:

A positive test for anti-GBM cannot be relied upon exclusively to establish the diagnosis of a disease mediated by GBM antibodies. Weakly positive test results may occur in other immune-mediated diseases, and renal or lung biopsy is often required to establish the diagnosis.

Methodology:

Multiplex Flow Immunoassay

References:

Mayo Medical Laboratories Web Page May 2013

Updates:

3/24/2006: Reference range change previously listed as ≤5.0 EU/mL (negative); 5.1 – 20.0 EU/mL (borderline); 20.1 – 400.0 EU/mL (positive)
11/18/2008: Reference range change at MML.
4/19/2011: Method change, previously listed as Enzyme Linked Immunosorbent Assay (ELISA) Enzyme Immunoassay (EIA).



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services