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Lab Dept:
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Serology
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Test Name:
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CMV ANTIBODIES, IgG & IgM
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General Information
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Lab Order Codes:
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CMV
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Synonyms:
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Anti-CMV Antibodies-IgG and IgM ; CMV Serology; Cytomegalovirus Antibodies
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CPT Codes:
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86644 – CMV antibodies (IgG)
86645 – CMV antibodies (IgM)
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Test Includes:
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Anti-CMV Antibodies, IgG and IgM
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Logistics
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Test Indications:
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Intended for the qualitative detection of IgG and IgM antibodies to Cytomegalovirus in human sera to indicate the following: no exposure to CMV or current or previous infection with CMV.
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Lab Testing Section:
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Chemistry (Performed on the St. Paul campus)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 3 days, testing is performed daily, Mon – Fri, during normal business hours
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top (plain, no gel) tube
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Draw Volume:
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1.5 mL blood
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Processed Volume:
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0.5 mL serum
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Collection:
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Blood should be collected aseptically by venipuncture and placed in a plain, red-top tube or other plain sterile tube without anticoagulant and allowed to clot at room temperature.
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Special Processing:
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Lab Staff: Centrifuge specimen as early as possible after specimen has clotted; transfer serum to a plain polypropylene tube and refrigerate. If testing is delayed longer than 48 hours, serum should be frozen at -20ºC or colder. Do not store serum in a self-defrosting freezer.
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Patient Preparation:
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None
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Sample Rejection:
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Sera exhibiting a high degree of hemolysis, icterus, lipemia or microbial contamination are not recommended because these conditions may cause aberrant results. Mislabeled or unlabeled specimens.
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Interpretive
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Reference Range:
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The results will be reported as a numerical value with interpretation.
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CMV–IgG (Result & Interpretation)
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CMV-IgM (Result & Interpretation)
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<0.6
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Negative
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<30
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Negative
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≥0.7
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Positive
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≥35
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Positive
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0.60 – 0.69
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Equivocal
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30 – 34.9
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Equivocal
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Expected values: Negative – No exposure to CMV. No detectable IgG or IgM antibodies present.
Interpretation:
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IgM Result
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IgG Result
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Interpretation
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Negative
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Negative
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Patient not exposed or too early
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Positive
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Negative or Positive
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Acute or recent infection
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Negative
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Positive
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Immunity from past exposure, not acute disease
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Equivocal Result: “Borderline result” – suggest repeat specimen be obtained in 7-14 days.
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Critical Values:
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N/A
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Limitations:
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Samples collected early in the course of a CMV infection may not have detectable levels of antibody. In such cases, it is recommended that a second serum sample be obtained 10-21 days later.
Positive results on CMV-IgG antibody in neonates should be interpreted with caution, since maternal IgG is transferred passively from mother to baby before birth. IgM assays are generally more useful indicators of infection in children below the age of 6 months.
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Methodology:
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Chemiluminescent Immunoassay (CLIA)
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References:
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Liaison® CMV IgG (310740) (February 2009) Directions for Use, DiaSorin, Inc., Stillwater, MN 55082
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Liaison® CMV IgM (310750) (February 2009) Directions for Use, DiaSorin, Inc., Stillwater, MN 55082
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NCCLS Guideline I/LA 18-A2 (September 2001) Specifications for Immunological Testing for Infectious Diseases; Approved Guideline-Second Edition, Vol 21, No 15
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Update:
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9/12/2005: Testing turnaround time previously listed as 1-6 days, testing performed 1 time per week.
8/29/2011: Testing method change, previously listed as ELISA. Note updated reference ranges.
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