Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

CMV ANTIBODIES, IgG & IgM

General Information

Lab Order Codes:

CMV

Synonyms:

Anti-CMV Antibodies-IgG and IgM ; CMV Serology; Cytomegalovirus Antibodies

CPT Codes:

86644 – CMV antibodies (IgG)
86645 – CMV antibodies (IgM)

Test Includes:

Anti-CMV Antibodies, IgG and IgM

Logistics

Test Indications:

Intended for the qualitative detection of IgG and IgM antibodies to Cytomegalovirus in human sera to indicate the following: no exposure to CMV or current or previous infection with CMV.

Lab Testing Section:

Chemistry (Performed on the St. Paul campus)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220--6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 3 days, testing is performed daily, Mon – Fri, during normal business hours

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top (plain, no gel) tube

Draw Volume:

1.5 mL blood

Processed Volume:

0.5 mL serum

Collection:

Blood should be collected aseptically by venipuncture and placed in a plain, red-top tube or other plain sterile tube without anticoagulant and allowed to clot at room temperature.

Special Processing:

Lab Staff: Centrifuge specimen as early as possible after specimen has clotted; transfer serum to a plain polypropylene tube and refrigerate. If testing is delayed longer than 48 hours, serum should be frozen at -20ºC or colder. Do not store serum in a self-defrosting freezer.

Patient Preparation:

None

Sample Rejection:

Sera exhibiting a high degree of hemolysis; icterus; lipemia or microbial contamination are not recommended because these conditions may cause aberrant results. Mislabeled or unlabeled specimens.

Interpretive

Reference Range:

The results will be reported as a numerical value with interpretation.

CMV–IgG (Result & Interpretation)

CMV-IgM (Result & Interpretation)

<0.6

Negative

<30

Negative

≥0.7

Positive

≥35

Positive

0.60 – 0.69

Equivocal

30 – 34.9

Equivocal

Expected values: Negative – No exposure to CMV. No detectable IgG or IgM antibodies present.

Interpretation:

IgM Result

IgG Result

Interpretation

Negative

Negative

Patient not exposed or too early

Positive

Negative or Positive

Acute or recent infection

Negative

Positive

Immunity from past exposure, not acute disease

Equivocal Result: “Borderline result” – suggest repeat specimen be obtained in 7-14 days.

Critical Values:

N/A

Limitations:

Samples collected early in the course of a CMV infection may not have detectable levels of antibody. In such cases, it is recommended that a second serum sample be obtained 10-21 days later.

Positive results on CMV-IgG antibody in neonates should be interpreted with caution, since maternal IgG is transferred passively from mother to baby before birth. IgM assays are generally more useful indicators of infection in children below the age of 6 months.

Methodology:

Chemiluminescent Immunoassay (CLIA)

References:

Liaison® CMV IgG (310740) (February 2009) Directions for Use, DiaSorin, Inc., Stillwater, MN 55082

Liaison® CMV IgM (310750) (February 2009) Directions for Use, DiaSorin, Inc., Stillwater, MN 55082

NCCLS Guideline I/LA 18-A2 (September 2001) Specifications for Immunological Testing for Infectious Diseases; Approved Guideline-Second Edition, Vol 21, No 15

Update:

9/12/2005: Testing turnaround time previously listed as 1-6 days, testing performed 1 time per week.
8/29/2011: Testing method change, previously listed as ELISA. Note updated reference ranges.



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