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Lab Dept:
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Serology
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Test Name:
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DOUBLE-STRANDED DNA ANTIBODY
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General Information
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Lab Order Codes:
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DSD
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Synonyms:
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DNA Double-Stranded (ds-DNA) Antibody, IgG, Serum; Anti-DNA; Anti-dsDNA; Anti- Native DNA; Double Stranded DNA; ds-DNA antibody; IgG Anti-DS DNA Antibody
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CPT Codes:
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86225 – Deoxyribonucleic acid (DNA) antibody; native or double stranded
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Test Includes:
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Double-stranded DNA antibody, IgG level reported in IU/mL.
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Logistics
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Test Indications:
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Useful in evaluating patients with signs and symptoms consistent with lupus erythematosus (LE). Monitoring patients with documented LE for flares in disease activity.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test: ADNA/8178)
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 3 days
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL (Minimum: 1.2 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.4 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot into screw-capped round bottom plastic vial. Store and ship specimen refrigerated. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Warm specimens: mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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All ages:
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<30.0 IU/mL
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Negative
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30.0 – 75.0 IU/mL
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Borderline
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>75.0 IU/mL
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Positive
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Negative is considered normal.
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Critical Values:
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N/A
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Limitations:
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Measurement of IgG antibodies to dsDNA are semiquantitative. Slight changes in the levels of these antibodies should not be relied upon to predict changes in clinical course of patients with LE. Clinical flares of disease in patients with LE may not be accompanied by changes in the levels of dsDNA antibodies. Positive results may occur in patients with diseases other than LE.
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Methodology:
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Enzyme-Linked Immunosorbent Assay
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References:
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Mayo Medical Laboratory Web Page March 2013
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Updates:
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4/18/2007: Reference ranges updated.
11/1/2007: Note Unit change from IU to IU/mL. Updated reference range due to change in method. Method previously listed as ELISA.
5/31/2011: Reference range update, ranges expanded by age. Method change previously listed as multiplex flow assay.
4/16/2013: Updated reference range.
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