Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

MONOSPOT

General Information

Lab Order Codes:

MON

Synonyms:

Infectious Mononucleosis Screen; Heterophile Screen

CPT Codes:

86308 – Heterophile antibodies; screening

Test Includes:

Monospot reported as positive or negative.

Logistics

Test Indications:

Useful as a screening test for the detection of heterophile antibodies related to infectious mononucleosis.

Lab Testing Sections:

Serology

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 hours

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Lavender top tube (EDTA)
Alternate: Red top tube, plain, no gel; Green top tube, (Li Heparin)

Draw Volume:

1.5 mL blood

Processed Volume:

0.5 mL plasma, serum or EDTA whole blood

Collection:

Routine venipuncture; Do Not use a serum separator tube

Special Processing:

Lab Staff: Centrifuge specimen, remove serum/plasma aliquot into a screw-capped round bottom plastic vial. Store refrigerated.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens

Interpretive

Reference Range:

Negative

Limitations:

In certain patients with clinical and hematological evidence of IM, specimen may give negative or weak results due to prozoning. Perform a diluted test when questioned about results by the patient’s physician.

Methodology:

The Biostar Acceava Mono II test is a rapid chromatographic immunoassay for the qualitative detection of infectious mononucleosis heterophile antibodies in whole blood, serum or plasma.

References:

Inverness Medical Bisostar Acceava Mono II, Test Package Insert 2006



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