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Lab Dept:
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Serology
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Test Name:
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MONOSPOT
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General Information
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Lab Order Codes:
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MON
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Synonyms:
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Infectious Mononucleosis Screen; Heterophile Screen
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CPT Codes:
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86308 – Heterophile antibodies; screening
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Test Includes:
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Monospot reported as positive or negative.
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Logistics
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Test Indications:
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Useful as a screening test for the detection of heterophile antibodies related to infectious mononucleosis.
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Lab Testing Sections:
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Serology
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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2 hours
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Lavender top tube (EDTA)
Alternate: Red top tube, plain, no gel; Green top tube, (Li Heparin)
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Draw Volume:
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1.5 mL blood
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Processed Volume:
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0.5 mL plasma or serum
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Collection:
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Routine venipuncture; Do Not use a serum separator tube
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum/plasma aliquot into a screw-capped round bottom plastic vial. Store refrigerated.
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Patient Preparation:
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None
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Sample Rejection:
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Mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Negative
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Limitations:
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In certain patients with clinical and hematological evidence of IM, specimen may give negative or weak results due to prozoning. Perform a diluted test when questioned about results by the patient’s physician.
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Methodology:
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Latex Agglutination
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References:
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Inverness Medical Bisostar Acceava Mono II, Test Package Insert 2006
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