Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

MITOCHONDRIAL ANTIBODIES

General Information

Lab Order Codes:

MIT

Synonyms:

Mitochondrial Antibodies (M2), Serum; Antimitochondrial antibodies; AMA; Immunology Profile

CPT Codes:

83516 – Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method

Test Includes:

Mitochondrial antibody level reported as Negative, Borderline, Weakly Positive or Positive.

Logistics

Test Indications:

Testing for Anti-Mitochondrial Antibodies (AMA) is indicated in patients suspected of having Primary Biliary Cirrhosis (PBC). Positive results for AMA of M2 specificity are highly specific for PBC, and false-negative results are rare.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test# 8176/AMA)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days, test set up Monday – Saturday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL (Minimum: 1.2 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.4 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot and store in a screw-capped plastic vial. Store and ship refrigerated. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than serum, gross hemolysis, warm specimens, gross lipemia, mislabeled or unlabeled specimens

Interpretive

Reference Range:

Negative

<0.1 Units

Borderline

0.1 - 0.3 Units

Weakly Positive

0.4 – 0.9 Units

Positive

≥1.0 Units

Note: Reference ranges apply to all ages.

Positive results for AMA of M2 specificity are highly specific for Primary Biliary Cirrhosis (PBC) and false-negative results are rare. A positive result for ANA of M2 specificity in patients with clinical features of PBC is virtually diagnostic for this disease.

Critical Values:

N/A

Limitations:

The level of AMA is not useful to indicate the stage or prognosis of the disease or for monitoring the course of the disease. Positive results are found (infrequently) in patients with CREST syndrome (Calcinosis Raynaud’s phenomenon, Esophageal hypomobility, Sclerodactyly, and Telangiectasia), relatives of patients with PBC and other autoimmune diseases.

Methodology:

Enzyme Immunosorbent Assay (EIA)

References:

Mayo Medical Laboratories June 2013

Updates:

5/12/2005: Mayo has expanded their reference range reporting for this test. Previously reported as Positive or Negative.

4/24/2006: Recommended storage temperature has changed from frozen to refrigerated. Frozen specimens will still be accepted, but refrigerated specimens have a faster turnaround time.



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