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Lab Dept:
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Serology
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Test Name:
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HELICOBACTER PYLORI IGG ANTIBODY
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General Information
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Lab Order Codes:
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HPYL
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Synonyms:
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H. Pylori IgG Antibody
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CPT Codes:
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86677 – Antibody; Helicobacter pylori
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Test Includes:
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Helicobacter pylori IgG antibody level
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Logistics
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Test Indications:
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Useful as a screening procedure to aid in the diagnosis of Helicobactor pylori infection in patients with active Helicobactor pylori associated gastritis.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories – New England (MML Test: HELIG)
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 3 days, test is performed Monday – Friday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL (Minimum: 1 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.25 mL) serum
Note: Submitting the minimum volume does not allow for repeat analysis and could result in a QNS (quantity not sufficient) result requiring a second specimen to be collected.
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and shop at refrigerated temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Gross hemolysis; gross lipemia; grossly icteric; heat inactivated serum; specimens other than serum; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Negative
Interpretation:
A positive result indicates the patient has antibodies to Helicobacter pylori. It does not necessarily indicate that any existing symptoms are due to Helicobacter pylori infection or colonization. It also does not differentiate between active and past infection.
IgG antibodies in the serum may remain elevated for months after successful eradication of Helicobacter pylori. Accordingly, if relapse is suspected, alternative testing including urea breath test, antigen detection in stool or endoscopic biopsy with stain, culture, and urease determination is suggested. If failure of treatment due to antimicrobial resistance is suspected, culture of biopsied specimens is required.
A negative result indicates that the patient does not have detectable levels of IgG antibody to Helicobacter pylori. However, if a specimen is drawn too early in Helicobacter pylori infection, IgG antibodies may not be present.
Because of the high predictive value of a negative result, an endoscopy procedure would not be indicated for the diagnosis of Helicobacter plyori infection. On the other hand, persistent symptoms after treatment of a seropositive patient may merit further evaluation.
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Critical Values:
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N/A
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Limitations:
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This assay should be performed only on patients with gastrointestinal symptoms because of the large percentage of H. pylori-colonized individuals, especially in older age groups (estimated to be 40-60% of asymptomatic Caucasians older than 60 years).
The serologic results must be interpreted in light of the clinical signs and symptoms of the patient.
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Methodology:
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Enzyme Immunoassay (EIA)
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References:
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Mayo Medical Laboratory Web Page March 2013
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Updates:
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11/23/2004: MML has changed the reference values from qualitative to quantitative results. Previously reported as positive or negative.
3/28/2013: Mayo Rochester no longer performing test with ELFA method as reagent manufacturer no longer supplying reagent. Testing moved to Mayo New England with similar EIA test reported as positive or negative.
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