Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

HELICOBACTER PYLORI IGG ANTIBODY

General Information

Lab Order Codes:

HPYL

Synonyms:

H. Pylori IgG Antibody

CPT Codes:

86677 – Antibody; Helicobacter pylori

Test Includes:

Helicobacter pylori IgG antibody level and Index Value

Logistics

Test Indications:

Useful as a screening procedure to aid in the diagnosis of Helicobactor pylori infection in patients with active Helicobactor pylori associated gastritis.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: SHELG)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days, test is performed Monday – Friday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL (Minimum: 1.2 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.4 mL) serum

Note: Submitting the minimum volume does not allow for repeat analysis and could result in a QNS (quantity not sufficient) result requiring a second specimen to be collected.

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and shop at refrigerated temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Gross hemolysis; gross lipemia; grossly icteric; heat inactivated serum; specimens other than serum; mislabeled or unlabeled specimens

Interpretive

Reference Range:

Negative (Results with Index values <8.95 are negative)

Interpretation:
A positive result indicates the patient has antibodies to Helicobacter pylori. It does not necessarily indicate that any existing symptoms are due to Helicobacter pylori infection or colonization. It also does not differentiate between active and past infection.

IgG antibodies in the serum may remain elevated for months after successful eradication of Helicobacter pylori. Accordingly, if relapse is suspected, alternative testing including urea breath test, antigen detection in stool or endoscopic biopsy with stain, culture, and urease determination is suggested. If failure of treatment due to antimicrobial resistance is suspected, culture of biopsied specimens is required.

A negative result indicates that the patient does not have detectable levels of IgG antibody to Helicobacter pylori. However, if a specimen is drawn too early in Helicobacter pylori infection, IgG antibodies may not be present.

Because of the high predictive value of a negative result, an endoscopy procedure would not be indicated for the diagnosis of Helicobacter plyori infection. On the other hand, persistent symptoms after treatment of a seropositive patient may merit further evaluation.

Critical Values:

N/A

Limitations:

This assay should be performed only on patients with gastrointestinal symptoms consistent with H. pylori infection and should not be used to test asymptomatic patients.

A positive test result does not distinguish between previous exposure, colonization or current infection with Helicobacter pylori. This test should be used as an aid to make the diagnosis alongside clinical evaluation and other laboratory tests.

Active disease due to H. pylori can be established by the urea breath test or the stool antigen assay.

Methodology:

Enzyme-Linked Immunosorbent assay (ELISA)

References:

Mayo Medical Laboratory Web Page August 2013

Updates:

11/23/2004: MML has changed the reference values from qualitative to quantitative results. Previously reported as positive or negative.
3/28/2013: Mayo Rochester no longer performing test with ELFA method as reagent manufacturer no longer supplying reagent. Testing moved to Mayo New England with similar EIA test reported as positive or negative.
8/27/2013: Mayo now performing the test in Rochester with new ELISA method and reporting an Index value. Note change in minimum draw/specimen requirements.



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services