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Lab Dept:
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Serology
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Test Name:
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LEPTOSPIRA ANTIBODY
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General Information
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Lab Order Codes:
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LEPA
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Synonyms:
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Leptospira Agglutination, Serum; Weil’s disease; Leptospirosis Antibody
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CPT Codes:
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86720 – Antibody; Leptospira
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Test Includes:
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Leptospira Antibody reported as negative or as a titer.
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Logistics
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Test Indications:
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As an aid in the diagnosis of leptosprirosis.
Known exposure to contaminated water. Usually abrupt onset with fever; may occur in 2 phases. Phase 1 has fever, chills, headache, muscle aches, vomiting or diarrhea. The patient may recover for a time but will become ill again. If a second phase occurs, it is more severe. The person may have kidney or liver failure or meningitis. This phase is also called Weil’s disease. Incubation range is 2 days–4 weeks.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 9697)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 5 days, test set-up daily
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL (Minimum: 0.5 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.15 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot. Store and ship at refrigerated temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Specimens other than serum, hemolysis, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Negative
Interpretation: Both IgM and IgG classes of antibody are produced in response to any of 16 leptospira serovars that produce infection. IgM antibody is first detectable within 1 to 2 weeks after onset of illness and peaks at 2 to 4 weeks.
Titers of 1:50 are considered borderline and follow-up specimens should be drawn for isolation of live leptospires (ie, culture) and repeat serology.
Titers >or =1:100 represent recent or active infection.
The overall sensitivity of the assay is 100% and the specificity is 97% when compared to the Centers for Disease Control and Prevention reagents.
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Critical Values:
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N/A
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Limitations:
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Serologic results must be correlated with the clinical picture. Sera drawn too close to onset of symptoms may precede the initial IgM antibody response. Acute titers may be delayed or substantially decreased by early and intensive antibiotic treatment. The assay detects antibody to the genus Leptospira but will not determine which serovar is associated with infection.
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Methodology:
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Indirect Hemagglutination
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References:
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Mayo Medical Laboratories February 2011
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Centers for Disease Control Web Page
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Updates:
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3/25/2004: Test moved from the Minnesota Department of Health to Mayo Medical Laboratories.
4/24/2008: Draw volumes temporarily increased for forward to ARUP.
2/17/2011: Testing performed internally at MML. Reference range and volume update.
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