Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

E. HISTOLYTICA ANTIBODY

General Information

Lab Order Codes:

EHAB

Synonyms:

E. Histolytica Antibody, Serum; Entamoeba; Amebic Serology; Amebiasis

CPT Codes:

86753 – Antibody; protozoa, not elsewhere specified

Test Includes:

Testing for E. Histolytica Antibody reported as positive or negative.

Logistics

Test Indications:

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess.

Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 9049/SAM)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 7 days; test set up Tuesday and Thursday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL (Minimum: 0.6 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.2 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot and freeze in a screw-capped, round bottom plastic vial. Ship frozen. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than serum; specimens received at ambient temperatures; mislabeled or unlabeled specimens; gross hemolysis; gross lipemia

Interpretive

Reference Range:

Negative

A positive enzyme-linked immunosorbent (ELISA) suggests current or previous infection with Entamoeba histolytica.

Since pathogenic and nonpathogenic species of entamoeba cannot be differentiated microscopically, some authorities believe a positive serology indicates the presence of the pathogenic species (ie, E. histolytica)

Critical Values:

N/A

Limitations:

Previous episodes of intestinal amebiasis may produce a positive serology.

Serologic results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.

Direct detection of Entamoeba histolytica in stool specimens is recommended to diagnose intestinal amebiasis.

Methodology:

Enzyme-Linked Immunoabsorbent Assay (ELISA)

References:

Mayo Medical Laboratories Web Page July 2013

Updates:

4/1/2010: MML will no longer perform titers on positive samples due to a change in kit design.
7/15/2010: Storage temp moved from frozen to refrigerated.
7/25/2013: Storage temp moved from refrigerated to frozen.



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