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Lab Dept:
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Serology
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Test Name:
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E. HISTOLYTICA ANTIBODY
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General Information
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Lab Order Codes:
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EHAB
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Synonyms:
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E. Histolytica Antibody, Serum; Entamoeba; Amebic Serology; Amebiasis
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CPT Codes:
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86753 – Antibody; protozoa, not elsewhere specified
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Test Includes:
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Testing for E. Histolytica Antibody reported as positive or negative.
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Logistics
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Test Indications:
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As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess.
Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 9049)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 7 days; test set up Tuesday and Thursday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL (Minimum: 0.6 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.2 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot and freeze in a screw-capped, round bottom plastic vial. Ship refrigerated. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Specimens other than serum, specimens received at ambient temperatures; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Negative
A positive enzyme-linked immunosorbent (ELISA) suggests current or previous infection with Entamoeba histolytica.
Since pathogenic and nonpathogenic species of entamoeba cannot be differentiated microscopically, some authorities believe a positive serology indicates the presence of the pathogenic species (ie, E. histolytica)
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Critical Values:
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N/A
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Limitations:
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Previous episodes of intestinal amebiasis may produce a positive serology.
Serologic results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.
Direct detection of Entamoeba histolytica in stool specimens is recommended to diagnose intestinal amebiasis.
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Methodology:
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Enzyme-Linked Immunoabsorbent Assay (ELISA)
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References:
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Mayo Medical Laboratories Web Page July 2010
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Updates:
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4/1/2010: MML will no longer perform titers on positive samples due to a change in kit design.
7/15/2010: Storage temp moved from frozen to refrigerated.
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