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Lab Dept:
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Serology
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Test Name:
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HLA TYPING (HLA CLASS I and II)
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General Information
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Lab Order Codes:
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HLA
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Synonyms:
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Class I and II Antigen Typing; Histocompatibility Testing; HLA Typing; Human Leukocyte Antigen Test; Tissue Typing; HLA – A, B, C, DR & DQ Typing, HLA Class I/II
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CPT Codes:
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83890 – Molecular diagnostics, molecular isolation or extraction
83898 x5 - Amplification of nucleic acid
83896 x100 – Nucleic acid probe each
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Test Includes:
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Serological definition of all “A, B, C, DR, DQ” or Class I and II HLA antigen specificities currently recognized by WHO (World Health Organization)
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Logistics
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Test Indications:
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Bone marrow transplantation, HLA disease association studies, Organ transplantation, Transplant workup
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Fairview-University Diagnostic Laboratory – Immunology/Histocompatibility
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Specimens are accepted Monday - Thursday, 24 hours; and on Fridays before 1:00 PM. Weekend collection is not recommended unless unavoidable.
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Turnaround Time:
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Testing performed Monday - Friday. Results are reported within 4 – 5 days.
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Special Instructions:
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Must include exact diagnosis: Solid Organ Recipient, Solid Organ Donor, Bone Marrow Recipient, or Bone Marrow Donor.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top tube
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Draw Volume:
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7 mL (Minimum: 2 mL) blood
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Processed Volume:
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Submit unprocessed whole blood in original Vacutainer®.
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Collection:
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Draw specimens according to the containers and amounts outlined above.
If the person being drawn is known to have a very low WBC count or is difficult to draw, contact the Laboratory to determine absolute minimum amounts of blood needed
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Special Processing:
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Lab Staff: Store and ship at room temperature. Do Not refrigerate or freeze. Specimen must arrive within 24 hours of collection Monday – Friday. Friday specimens should be at Fairview University by 1300. Do Not ship specimens on Friday or Saturday.
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Patient Preparation:
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Collect specimens prior to dialysis.
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Sample Rejection:
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Frozen, refrigerated, or clotted specimen
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Interpretive
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Reference Range:
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Interpretive report generated
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Molecular PCR/SSOP typing
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References:
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Fairview University Diagnostic Laboratory Web Page http://labguide.fairview.org/diagnostic.asp February 2005
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Changes:
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11/21/03: CPT codes updated due to method change, Method changed to Molecular typing, previously listed as cytoxicity.
3/1/2005: Changed tube type requirement, previously listed as yellow or green. Updated CPT coding for 2005 standards. Adjusted draw volume previously listed as 20 mL (Minimum: 10 mL). Test description changed to reflect DQ and DR typings as being included in the testing.
5/12/2010: CPT codes updated.
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