Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

HIV 1 ANTIBODY CONFIRMATION BY WESTERN BLOT

General Information

Lab Order Codes:

HIVCO

Synonyms:

W. Blot Anti-HIV; HTLV III; AIDS; Aquired Immune Deficiency Syndrome; HIV1 Antibody

CPT Codes:

86689 – Antibody; HTLV or HIV antibody, confirmatory test

Test Includes:

HIV-1 Western Blot (WB) analysis reported as Negative, Positive or Indeterminate. See HIV Serologic Screening Algorithm for more information.

Logistics

Test Indications:

Supplemental serologic testing for HIV-1 infection in patients with indeterminate or reactive HIV-1 antibody results (regardless of HIV-2 antibody result) by FDA-approved HIV-1/2 antibody differentiation test.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: HV1WB)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

4.8 mL (Minimum: 1.2 mL) blood

Processed Volume:

1.6 mL (Minimum: 0.4 mL) serum

Collection:

Routine venipuncture

Special Processing:

N/A

Patient Preparation:

Lab Staff: Centrifuge specimen, remove serum aliquot and send specimen in a plastic, screw top tube. Send specimen frozen. Forward promptly.

Sample Rejection:

Mislabeled or unlabeled specimens, specimens other than serum

Interpretive

Reference Range:

Negative.

Critical Values:

N/A

Limitations:

The US Association of Public Health Laboratories recommends verification of all first-time positive confirmatory test results prior to the diagnosis of HIV infection. A second serum should be obtained from the patient and submitted for repeat testing to verify all positive results.

Positive HIV antibody WB results in infants of <18 months of age and born to HIV-infected mothers may indicate passive transfer of maternal HIV antibodies. Serologic tests (screening, supplemental or confirmatory) cannot distinguish between active neonatal HIV infection and passive transfer of maternal HIV antibodies in infants during the postnatal period (up to 18 months). Diagnosis of HIV infection in newborns and infants should be made by virologic tests such as detection of HIV RNA or HIV proviral DNA.

This test should be ordered only on sera that are either indeterminate or reactive for HIV-1 antibodies (regardless of the HIV-2 antibody result) by FDA approved HIV-1/2 antibody differentiation tests.

Although hemolyzed serum specimens are acceptable for testing, this assay is not FDA-approved for testing cadaveric serum specimens.

Performance characteristics have not been established for the following specimen types: Cadaveric specimens, Specimens containing particulate matter

Methodology:

HIV-1 WB: Western Blot

References:

Mayo Medical Laboratories November 2014

Updates:

4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories. Note: Test now reflexes to supplemental/confirmatory testing (with additional charges) when indicated by reactive findings.
9/27/2010: Specimen requirement updates.



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