Lab Dept:

Serology

Test Name:

POLIOVIRUS TYPES 1, 2, 3 ANTIBODY

General Information

Lab Order Codes:

PVIS

Synonyms:

Poliovirus Titer, CF, Total Antibody; Poliovirus Antibody by Complement Fixation

CPT Codes:

86658 x3 – Antibody; enterovirus

Test Includes:

Poliovirus Type 1, 2 & 3 Total Antibody reported as a titer.

Logistics

Test Indications:

To support the diagnosis of acute poliovirus infection, documentation of previous exposure to poliovirus

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories forward to Focus Technologies (MML Test: POLIO)

Phone Numbers:

Minneapolis:

Saint Paul:

612-813-6280

651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 - 5 days, test set-up Sunday – Thursday.

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

3 mL blood

Processed Volume:

1 mL (Minimum: 0.5 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at refrigerated temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimen

Interpretive

Reference Range:

<1:8 Antibody not detected

≥1:8 Antibody detected

This assay is designed to detect recent poliovirus infection; single titers > or = provide strong support for recent natural (non-vaccine) exposure. The test is not appropriate for assessing vaccine responses or immunity to polioviruses; poliovirus neutralization is the recommended test for these purposes.

Critical Values:

N/A

Limitations:

N/A

Methodology:

Complement fixation (CF)

References:

Mayo Medical Laboratories Web Page August 2012

Focus Technologies Web Page August 2012

Updates:

3/2/2004: Test moved form Lab Corp to MML (forward to Focus Laboratory). Test previously measured IgG antibody and now measure total antibody.
8/20/12: Updated MML order code and reference range information.