Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

HEPATITIS C ANTIBODY CONFIRMATION

General Information

Lab Order Codes:

HCRIB

Synonyms:

HCV RIBA; Hepatitis C Confirmation; Anti-HCV Confirmation

CPT Codes:

86804 – Hepatitis C antibody, confirmatory test

Test Includes:

This test is a supplemental test for positive HCV antibody chemiluminescence immunoassay (CIA) result. It will be reported as positive, negative or indeterminate. If the patient has tested positive elsewhere by another screening method, this test can be ordered individually as the confirmatory test.

Logistics

Test Indications:

Intended for use as an additional, more specific test on human serum or plasma specimens found to be repeatedly reactive using a licensed anti-HCV screening procedure, such as enzyme-linked immunosorbent assay (ELISA). Possible confirmatory tests include direct detection of HCV RNA by the reverse transcriptase-polymerase chain reaction (RT-PCR), which is the most sensitive and specific technique available, and recombinant immunoblot assay (RIBA), which is a supplemental test for HCV antibodies.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (Test# 80181)

Phone Numbers:

Minneapolis:

Saint Paul:

 

612-813-6280

651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 – 5 days, test performed Monday – Saturday.

Special Instructions:

This test is not offered as a screening or confirmatory test for blood donor specimens. This assay is not US Food and Drug Administration approved for testing cadaveric or hemolyzed specimens.

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL blood

Processed Volume:

0.5 mL (Minimum: 0.1 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum from clot within 1 hour. Serum aliquot should be placed in a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than serum or plasma, warm specimens, mislabeled or unlabeled specimens.

Interpretive

Reference Range:

Negative (reported as positive, negative or indeterminate. Bands detected are included in all positive and indeterminate reports).

Interpretation depends on clinical setting.

Critical Values:

N/A

Limitations:

Not useful for detection of early acute hepatitis C infection. Not useful for differentiation between resolved and chronic hepatitis C infections. Performance characteristics have not been established for the following specimen types: hemolysis, specimens containing particulate matter.

Methodology:

Recombinant Immunoblot Assay (RIBA)

References:

Mayo Medical Laboratories Web Page April 2011

Updates:

4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories.
11/12/2007: Name change removing RIBA and replacing it with confirmation.



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