Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



Anti-HIV-2 Confirmation; Anti-HIV-2 Western Blot

CPT Codes:

86689 – HIV confirmatory test

Test Includes:

HIV-2 Antibody Immunoblot reported as negative or positive.

Note: Test is automatically a part of the HIV-1,2 Antibody Evaluation Reflex and does not need to be ordered separately if the Evaluation has been ordered. A direct order of this test should only be necessary for repeats and confirmations or when initial testing has been done elsewhere.


Test Indications:

Confirmation of HIV-2 antibody.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories forward to Focus Technologies, Inc. (Test: 80443)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 6 days, test performed Tuesday and Thursday

Special Instructions:

Refer to the Patient HIV Testing Algorithm for more information.

This assay utilizes reagents labeled “For Research Use Only” by the manufacturer; it has not been approved by the FDA. If used in a blood bank setting, it is the responsibility of the client to follow all applicable FDA regulations and guidelines. The assay’s performance characteristics have been established by Focus Technologies, Inc.


Specimen Type:

Whole blood


Red top tube

Draw Volume:

3 mL (Minimum: 1 mL) blood

Processed Volume:

1 mL (Minimum: 0.25 mL) serum


Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:


Sample Rejection:

Specimens other than serum


Reference Range:

Negative (reported as negative or positive)

HIV-2, a retrovirus related to HIV-1, can cause immunosuppression and AIDS. The CDC (MMWR 41:rr12, 1992) recommends only specimens repeatedly reactive on combination HIV-1,2 EIA assay, negative or indeterminate on HIV-1 Western blot, and positive on HIV-2 EIA be tested by HIV-2 Immunoblot. An exception to this recommendation is a positive result by HIV-1 blot in a patient with risk factors for HIV-2 infection. Due to the strong serologic cross-reaction between HIV-1 and HIV-2, it is essential that all specimens sent for HIV-2 Immunoblot also have HIV-1 Western blot results available (including the bands detected) in order to meaningfully interpret the HIV-2 Immunoblot results.

Critical Values:







Mayo Medical Laboratories Web Page

Focus Technologies, Inc. Web Page

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