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Lab Dept:
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Serology
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Test Name:
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HIV-2 ANTIBODY IMMUNOBLOT CONFIRMATION (ANTI-HIV-2 CONFIRMATION)
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General Information
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Lab Order Codes:
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H2WWB
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Synonyms:
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Anti-HIV-2 Confirmation; Anti-HIV-2 Western Blot
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CPT Codes:
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86689 – HIV confirmatory test
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Test Includes:
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HIV-2 Antibody Immunoblot reported as negative or positive.
Note: Test is automatically a part of the HIV-1,2 Antibody Evaluation Reflex and does not need to be ordered separately if the Evaluation has been ordered. A direct order of this test should only be necessary for repeats and confirmations or when initial testing has been done elsewhere.
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Logistics
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Test Indications:
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Confirmation of HIV-2 antibody.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories forward to Focus Technologies, Inc. (Test# 80443)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 6 days, test performed Tuesday and Thursday
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Special Instructions:
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Refer to the Patient HIV Testing Algorithm for more information.
This assay utilizes reagents labeled “For Research Use Only” by the manufacturer; it has not been approved by the FDA. If used in a blood bank setting, it is the responsibility of the client to follow all applicable FDA regulations and guidelines. The assay’s performance characteristics have been established by Focus Technologies, Inc.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Red top tube
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Draw Volume:
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3.0 mL blood
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Processed Volume:
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1.0 mL (Minimum: 0.25 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Specimens other than serum
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Interpretive
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Reference Range:
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Negative (reported as negative or positive)
HIV-2, a retrovirus related to HIV-1, can cause immunosuppression and AIDS. The CDC (MMWR 41:rr12, 1992) recommends only specimens repeatedly reactive on combination HIV-1,2 EIA assay, negative or indeterminate on HIV-1 Western blot, and positive on HIV-2 EIA be tested by HIV-2 Immunoblot. An exception to this recommendation is a positive result by HIV-1 blot in a patient with risk factors for HIV-2 infection. Due to the strong serologic cross-reaction between HIV-1 and HIV-2, it is essential that all specimens sent for HIV-2 Immunoblot also have HIV-1 Western blot results available (including the bands detected) in order to meaningfully interpret the HIV-2 Immunoblot results.
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Immunoblot
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Contraindications:
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N/A
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References:
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Mayo Medical Laboratories Web Page http://www.mayomedicallaboratories.com/
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Focus Technologies, Inc. Web Page www.focustechnologies.com
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