|
|
|
Lab Dept:
|
Serology
|
Test Name:
|
GLIADIN, DEAMIDATED, IGA ANTIBODY
|
General Information
|
Lab Order Codes:
|
GLA
|
Synonyms:
|
Deamidated Gliadin IgA Antibody
|
CPT Codes:
|
83516 – Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method
|
Test Includes:
|
Deamidated Glidadin IgA Antibody level reported in Elia U/mL.
|
Logistics
|
Test Indications:
|
Evaluating patients suspected of having celiac disease, including patients with symptoms compatible with celiac disease, patients with atypical symptoms, and individuals at increased risk of celiac disease.
Monitoring and compliance with a gluten-free diet.
|
Lab Testing Sections:
|
Chemistry – Minneapolis Campus only
|
Phone Numbers:
|
MIN Lab: 612-813-6280
|
STP Lab: 651-220-6550
|
Test Availability:
|
Daily, 24 hours
|
Turnaround Time:
|
1 - 4 days, test performed Monday - Friday
|
Special Instructions:
|
N/A
|
Specimen
|
Specimen Type:
|
Blood
|
Container:
|
Red top tube
|
Draw Volume:
|
1 mL (Minimum: 0.6 mL) blood
|
Processed Volume:
|
0.3 mL (Minimum: 0.2 mL) serum
|
Collection:
|
Routine venipuncture
|
Special Processing:
|
Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.
|
Patient Preparation:
|
None
|
Sample Rejection:
|
Mislabeled or unlabeled specimens
|
Interpretive
|
Reference Range:
|
Deamidated Gliadin IgA (AGP) Antibodies (all ages)
|
<7 Elia U/mL
|
Negative
|
7 – 10 Elia U/mL
|
Equivocal
|
>10 Elia U/mL
|
Positive
|
Interpretation:
AGP >10 Elia U/mL or AGP and GGP (Deamidated Gliadin IgG) >10 Elia U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy
|
Critical Values:
|
N/A
|
Limitations:
|
N/A
|
Methodology:
|
Fluoroenzyme immunoassay
|
References:
|
Phadia AB Elia Directions for Use, May 2010
|
CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition
|
Updates:
|
10/9/2007: Mayo implemented the use of an ELISA assay that detects deamidated gliadin. Note new reference ranges.
11/2/2009: Testing moved from MML to testing internally at Children’s.
5/13/2013: Move from Gliadin IgA to Deamidated Gliadin IgA.
|
