Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

GLIADIN, DEAMIDATED, IGA ANTIBODY

General Information

Lab Order Codes:

GLA

Synonyms:

Deamidated Gliadin IgA Antibody

CPT Codes:

83516 – Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method

Test Includes:

Deamidated Glidadin IgA Antibody level reported in Elia U/mL.

Logistics

Test Indications:

Evaluating patients suspected of having celiac disease, including patients with symptoms compatible with celiac disease, patients with atypical symptoms, and individuals at increased risk of celiac disease.

Monitoring and compliance with a gluten-free diet.

Lab Testing Sections:

Chemistry – Minneapolis Campus only

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 4 days, test performed Monday - Friday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1 mL (Minimum: 0.6 mL) blood

Processed Volume:

0.3 mL (Minimum: 0.2 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens

Interpretive

Reference Range:

Deamidated Gliadin IgA (AGP) Antibodies (all ages)

<7 Elia U/mL

Negative

7 – 10 Elia U/mL

Equivocal

>10 Elia U/mL

Positive

Interpretation:
AGP >10 Elia U/mL or AGP and GGP (Deamidated Gliadin IgG) >10 Elia U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy

Critical Values:

N/A

Limitations:

N/A

Methodology:

Fluoroenzyme immunoassay

References:

Phadia AB Elia Directions for Use, May 2010

CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition

Updates:

10/9/2007: Mayo implemented the use of an ELISA assay that detects deamidated gliadin. Note new reference ranges.
11/2/2009: Testing moved from MML to testing internally at Children’s.
5/13/2013: Move from Gliadin IgA to Deamidated Gliadin IgA.



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