Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

GLIADIN, DEAMIDATED, IGG ANTIBODY

General Information

Lab Order Codes:

GGP

Synonyms:

Deamidated Gliadin IgG Antibody

CPT Codes:

83516 - Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method

Test Includes:

Deamidated Gliadin IgG Antibody reported in EliA U/mL.

Logistics

Test Indications:

Evaluating patients suspected of having celiac disease, including patients with symptoms compatible with celiac disease, patients with atypical symptoms, and individuals at increased risk of celiac disease.

Monitoring compliance with a gluten-free diet.

Lab Testing Sections:

Chemistry – Minneapolis Campus only

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 4 days, performed Monday - Friday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1 mL (Minimum: 0.6 mL) blood

Processed Volume:

0.3 mL (Minimum: 0.2 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot and transfer into a screw-capped plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.

Stability: Refrigerated up to 48 hours, room temperature for 8 hours.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens; lipemic; hemolysis; contaminated specimens

Interpretive

Reference Range:

Deamidated Gliadin IgG (GGP) Antibodies (all ages)

<7 EliA U/mL

Negative

7 – 10 EliA U/mL

Equivocal

>10 EliA U/mL

Positive

Interpretation:
Gliadin, Deamidated, IgA >10 EliA U/mL or Gliadin, Deamidated, IgA >10 and GGP >10 EliA U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy

Critical Values:

N/A

Limitations:

N/A

Methodology:

Fluoroenzyme immunoassay

References:

Phadia AB EliA Directions for Use (August 2010)

CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition

Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition, 2001

Updates:

10/9/2007: Mayo implemented the use of an ELISA assay that detects deamidated gliadin. Note new reference ranges.
11/2/2009: Testing moved from MML to testing internally at Children’s.
5/15/2013: Move from Gliadin IgG to Deamidated Gliadin IgG. Note reference value changes.
8/2/2015: Sample rejection criteria update.



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