Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

HIV-1 ANTIBODY (ANTI-HIV-1) IMMUNOFLUORESCENCE

General Information

Lab Order Codes:

Not orderable as an individual test, part of the HIV 1,2 Evaluation Reflex as a confirmatory test. If there is a need to order this test individually, please contact the laboratory.

Synonyms:

HIV-1 Antibody; Anti-HIV-1; HIV-1 Screen; HIV-1 Immunofluorescence

CPT Codes:

86689 – HIV-1 confirmatory test

Test Includes:

HIV-1 antibody reported as positive, negative or indeterminate.

Logistics

Test Indications:

The HIV-1 indirect immunofluorescence assay (IFA) is an in vitro, qualitative assay for the detection of antibodies to HIV-1 in human serum or plasma. It is intended to be used as an additional, more specific test for antibodies to HIV-1 in specimens found to be repeatedly reactive by screening procedures, such as enzyme immunoassay. Initially, it will be used to reconcile indeterminate HIV-1 western blots and as an additionally supplementary test when a diagnostic problem exists.

Note: Test is automatically a part of the HIV-1,2 Antibody Evaluation Reflex and does not need to be ordered separately if the Evaluation has been ordered. A direct order of this test should only be necessary for repeats and confirmations or when initial testing has been done elsewhere.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 81758)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 2 days

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood

Container:

Red top tube

Draw Volume:

1.5 mL blood

Processed Volume:

0.5 mL (Minimum: 0.2 mL) serum

Collection:

Routine venipuncture

Special Processing:

N/A

Patient Preparation:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Sample Rejection:

Mislabeled or unlabeled specimens

Interpretive

Reference Range:

Negative (reported as negative, positive or indeterminate)

A negative result indicates the absence of HIV-1 specific antibodies.

A positive result indicates the presence of HIV-1 specific antibodies.

An indeterminate result does not imply that HIV-1 antibodies are, or are not present in the test specimen. It indicates that HIV-1 status of the specimen cannot be resolved through the use of the HIV-1 IFA assay. The correct evaluation in such situations must be based on subsequent repeat testing and clinical evaluation.

Critical Values:

N/A

Limitations:

Some individuals infected with HIV-2 may have antibodies that recognize and bind to viral agents present on the HIV-1 IFA slides.

Gross microbial contamination, hemolysis, lipemia or gelatination of serum should be avoided. Samples from patients with jaundice may cause non-specific results.

Insufficient data are available to support use of the HIV-1 IFA with any body fluids other than serum/plasma or processed plasma.

Test specimens should not be subjected to freezing and thawing more than twice.

Performance characteristics have not been established for the following specimen characteristics: hemolysis, specimens containing particulate matter.

Methodology:

Immunofluorescence Assay (IFA)

References:

Mayo Medical Laboratory Web Page November 2005

Updates:

4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories. Note: Change in methodology from ELISA to Immunofluorescence (IFA) and change in CPT code from 86701 to 86689.



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