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Lab Dept:
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Serology
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Test Name:
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HIV-1 ANTIBODY (ANTI-HIV-1) IMMUNOFLUORESCENCE
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General Information
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Lab Order Codes:
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Not orderable as an individual test, part of the HIV 1,2 Evaluation Reflex as a confirmatory test. If there is a need to order this test individually, please contact the laboratory.
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Synonyms:
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HIV-1 Antibody; Anti-HIV-1; HIV-1 Screen; HIV-1 Immunofluorescence
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CPT Codes:
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86689 – HIV-1 confirmatory test
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Test Includes:
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HIV-1 antibody reported as positive, negative or indeterminate.
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Logistics
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Test Indications:
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The HIV-1 indirect immunofluorescence assay (IFA) is an in vitro, qualitative assay for the detection of antibodies to HIV-1 in human serum or plasma. It is intended to be used as an additional, more specific test for antibodies to HIV-1 in specimens found to be repeatedly reactive by screening procedures, such as enzyme immunoassay. Initially, it will be used to reconcile indeterminate HIV-1 western blots and as an additionally supplementary test when a diagnostic problem exists.
Note: Test is automatically a part of the HIV-1,2 Antibody Evaluation Reflex and does not need to be ordered separately if the Evaluation has been ordered. A direct order of this test should only be necessary for repeats and confirmations or when initial testing has been done elsewhere.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 81758)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 2 days
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL blood
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Processed Volume:
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0.5 mL (Minimum: 0.2 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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N/A
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Patient Preparation:
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Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.
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Sample Rejection:
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N/A
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Interpretive
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Reference Range:
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Negative (reported as negative, positive or indeterminate)
A negative result indicates the absence of HIV-1 specific antibodies.
A positive result indicates the presence of HIV-1 specific antibodies.
An indeterminate result does not imply that HIV-1 antibodies are, or are not present in the test specimen. It indicates that HIV-1 status of the specimen cannot be resolved through the use of the HIV-1 IFA assay. The correct evaluation in such situations must be based on subsequent repeat testing and clinical evaluation.
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Critical Values:
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N/A
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Limitations:
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Some individuals infected with HIV-2 may have antibodies that recognize and bind to viral agents present on the HIV-1 IFA slides.
Gross microbial contamination, hemolysis, lipemia or gelatination of serum should be avoided. Samples from patients with jaundice may cause non-specific results.
Insufficient data are available to support use of the HIV-1 IFA with any body fluids other than serum/plasma or processed plasma.
Test specimens should not be subjected to freezing and thawing more than twice.
Performance characteristics have not been established for the following specimen characteristics: hemolysis, specimens containing particulate matter.
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Methodology:
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Immunofluorescence Assay (IFA)
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Contraindications:
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N/A
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References:
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Mayo Medical Laboratory Web Page http://www.mayoreferenceservices.org/mrs/mml/index.asp November 2005
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Updates:
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4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories. Note: Change in methodology from ELISA to Immunofluorescence (IFA) and change in CPT code from 86701 to 86689.
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