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Lab Dept:
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Serology
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Test Name:
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CELIAC DISEASE PANEL REFLEX
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General Information
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Lab Order Codes:
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CELIA
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Synonyms:
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Celiac Disease Panel
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CPT Codes:
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83516 x4 – Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method
82784 – Total IgA (if appropriate)
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Test Includes:
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Anti-Deamidated Gliadin IgA Specific, Anti-Deamidated Gliadin IgG Specific, Anti-Tissue Transglutaminase IgA Specific, Anti-Tissue Transglutaminase IgG Specific; TTGA; TTGG
Reflex: Total IgA will be performed at an additional charge when tTG-IgA is negative, and tTG-IgG is positive
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Logistics
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Test Indications:
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Useful for differential diagnosis of intestinal disorders such as celiac disease and Crohn’s disease. Also useful for monitoring a gluten-free diet.
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Lab Testing Sections:
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Chemistry – Minneapolis campus only
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 4 days; performed Tuesday & Friday
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Special Instructions:
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Endomysial Antibody is orderable separately and referred to Mayo Medical Laboratories for testing.
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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1.5 mL (Minimum: 0.9 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.3 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-top plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.
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Patient Preparation:
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None
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Sample Rejection:
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Mislabeled or unlabeled specimen
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Interpretive
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Reference Range:
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Test Name
(all ages)
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Negative
(Elia U/mL)
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Equivocal
(Elia U/mL)
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Positive
(Elia U/mL)
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Tissue transglutaminase IgA (TTGA)
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<7
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7 - 10
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>10
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Tissue transglutaminase IgG (TTGG)
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<7
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7 - 10
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>10
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Deamidated Gliadin antibody IgA (GGP)
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<7
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7 - 10
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>10
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Deamidated Gliadin antibody IgG (AGP)
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<7
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>10
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Reflexive Total IgA (mg/dL)
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Please refer to IgA (Immunoglobulin A) for reference ranges.
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Interpretation:
TTGA >10 Elia U/mL: Suggestive of Celiac disease
TTGA and TTGG both >10 U/mL: Suggestive of Celiac disease
AGP >10 Elia U/mL or AGP and GLG >10 Elia U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Fluorenzyme immunoassay
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References:
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Phadia AB Elia Directions for Use (August 2010)
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CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition
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Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition
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Updates:
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1/11/2007: CPT 2007 update
10/9/2007: Gliadin IgG and IgA updated to deamidated ELISA tests.
10/14/2008: tTG reference range/units update previously listed as <20 Units.
11/2/2009: Testing moved from MML to testing internally at Children’s.
2/2/2012: Gliadin Ab IgG reference range updated.
5/15/2013: Gliadin IgG and IgA changed to Deamidated Gliadin IgG and IgA.
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