Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

CELIAC DISEASE PANEL REFLEX

General Information

Lab Order Codes:

CELIA

Synonyms:

Celiac Disease Panel

CPT Codes:

83516 x4 – Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method

82784 – Total IgA (if appropriate)

Test Includes:

Anti-Deamidated Gliadin IgA Specific, Anti-Deamidated Gliadin IgG Specific, Anti-Tissue Transglutaminase IgA Specific, Anti-Tissue Transglutaminase IgG Specific; TTGA; TTGG

Reflex: Total IgA will be performed at an additional charge when tTG-IgA is negative, and tTG-IgG is positive

Logistics

Test Indications:

Useful for differential diagnosis of intestinal disorders such as celiac disease and Crohn’s disease. Also useful for monitoring a gluten-free diet.

Lab Testing Sections:

Chemistry – Minneapolis campus only

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 4 days; performed Tuesday & Friday

Special Instructions:

Endomysial Antibody is orderable separately and referred to Mayo Medical Laboratories for testing.

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL (Minimum: 0.9 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.3 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-top plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimen

Interpretive

Reference Range:

Test Name
(all ages)

Negative
(Elia U/mL)

Equivocal
(Elia U/mL)

Positive
(Elia U/mL)

Tissue transglutaminase IgA (TTGA)

<7

7 - 10

>10

Tissue transglutaminase IgG (TTGG)

<7

7 - 10

>10

Deamidated Gliadin antibody IgA (GGP)

<7

7 - 10

>10

Deamidated Gliadin antibody IgG (AGP)

<7

 

>10

Reflexive Total IgA (mg/dL)

Please refer to IgA (Immunoglobulin A) for reference ranges.

Interpretation:
TTGA >10 Elia U/mL: Suggestive of Celiac disease
TTGA and TTGG both >10 U/mL: Suggestive of Celiac disease
AGP >10 Elia U/mL or AGP and GLG >10 Elia U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy

Critical Values:

N/A

Limitations:

N/A

Methodology:

Fluorenzyme immunoassay

References:

Phadia AB Elia Directions for Use (August 2010)

CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition

Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition

Updates:

1/11/2007: CPT 2007 update
10/9/2007: Gliadin IgG and IgA updated to deamidated ELISA tests.
10/14/2008: tTG reference range/units update previously listed as <20 Units.
11/2/2009: Testing moved from MML to testing internally at Children’s.
2/2/2012: Gliadin Ab IgG reference range updated.
5/15/2013: Gliadin IgG and IgA changed to Deamidated Gliadin IgG and IgA.



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services