|
|
Lab Dept:
|
Serology
|
Test Name:
|
LEGIONELLA PNEUMOPHILA ANTIBODY
|
General Information
|
Lab Order Codes:
|
LEGI
|
Synonyms:
|
LDA (Legionnaires’ Disease Antibody); Legionnaire’s Disease
|
CPT Codes:
|
86713 – Antibody, Legionella
|
Test Includes:
|
Detection of Legionella pneumophila antibody reported as positive or negative.
|
Logistics
|
Test Indications:
|
Evaluation of possible legionellosis (Legionnaires’ Disease, Pontiac Fever, extrapulmonary legionella infection caused by Legionella pneumophila).
|
Lab Testing Sections:
|
Serology - Sendouts
|
Referred to:
|
Lab Corp/Viromed (Test# 164616)
|
Phone Numbers:
Minneapolis:
Saint Paul:
|
|
612-813-6280
|
651-220-6550
|
Test Availability:
|
Daily, 24 hours
|
Turnaround Time:
|
2 - 4 days
|
Special Instructions:
|
Paired specimens, both acute and convalescent (3-6 weeks later) sera are strongly recommended. Please label specimens as acute or convalescent.
|
Specimen
|
Specimen Type:
|
Whole blood
|
Container:
|
Red top tube
|
Draw Volume:
|
3.0 mL (Minimum: 0.6 mL) blood per specimen
|
Processed Volume:
|
1.0 mL (0.6 mL) serum per specimen
|
Collection:
|
Routine venipuncture
|
Special Processing:
|
Lab Staff: Centrifuge specimen. Remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.
Note:
● Paired specimens, both acute and convalescent sera are strongly recommended.
● Label specimens appropriately (ie, unpaired: acute or convalescent or paired: paired acute and paired convalescent.)
● If paired specimens are submitted, separate accession numbers are required.
● Appropriately labeled paired specimens received together will be analyzed on the same run and charged separately.
● Specimens sent separately will be run and charged separately.
|
Patient Preparation:
|
None
|
Sample Rejection:
|
Specimens other than serum, mislabeled or unlabeled specimens, warm specimens
|
Interpretive
|
Reference Range:
|
The positive cutoff has been set to the equivalent for an IFA titer of 1:256. If only one specimen is submitted, a positive is presumptive supporting evidence of exposure.
|
Critical Values:
|
N/A
|
Limitations:
|
This system tests for Legionella pneumophila serogroups 1-6. It does not test for nonpneumophila, or atypical, Legionella species. A diagnosis should not be made on the basis of anit-Legionella serology results alone; test results should be interpreted in conjunction with the clinical evaluation and other diagnostic procedures.
|
Methodology:
|
Enzyme-linked Immunosorbent Assay (ELISA)
|
Contraindications:
|
N/A
|
References:
|
Lab Corp Web Page January 2007
|
