Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

LEGIONELLA PNEUMOPHILA ANTIBODY

General Information

Lab Order Codes:

LEGI

Synonyms:

LDA (Legionnaires’ Disease Antibody); Legionnaire’s Disease

CPT Codes:

86713 – Antibody, Legionella

Test Includes:

Detection of Legionella pneumophila antibody reported as positive or negative.

Logistics

Test Indications:

Evaluation of possible legionellosis (Legionnaires’ Disease, Pontiac Fever, extrapulmonary legionella infection caused by Legionella pneumophila).

Lab Testing Sections:

Serology - Sendouts

Referred to:

Lab Corp/Viromed (Test#: 164616)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 - 4 days

Special Instructions:

Paired specimens, both acute and convalescent (3-6 weeks later) sera are strongly recommended. Please label specimens as acute or convalescent.

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.2 mL (Minimum: 0.6 mL) blood per specimen

Processed Volume:

0.4 mL (0.2 mL) serum per specimen

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen. Remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at ambient temperatures. Forward promptly.

Note:
● Paired specimens, both acute and convalescent sera are strongly recommended.
● Label specimens appropriately (ie, unpaired: acute or convalescent or paired: paired acute and paired convalescent.)
● If paired specimens are submitted, separate accession numbers are required.
● Appropriately labeled paired specimens received together will be analyzed on the same run and charged separately.
● Specimens sent separately will be run and charged separately.

Patient Preparation:

None

Sample Rejection:

Specimens other than serum; mislabeled or unlabeled specimens; hemolysis; lipemia; gross bacterial contamination

Interpretive

Reference Range:

The positive cutoff has been set to the equivalent for an IFA titer of 1:256. If only one specimen is submitted, a positive is presumptive supporting evidence of exposure.

Critical Values:

N/A

Limitations:

This system tests for Legionella pneumophila serogroups 1-6. It does not test for non-pneumophila, or atypical, Legionella species. A diagnosis should not be made on the basis of anit-Legionella serology results alone; test results should be interpreted in conjunction with the clinical evaluation and other diagnostic procedures.

Methodology:

Enzyme-linked Immunosorbent Assay (ELISA)

References:

Lab Corp Web Page July 2013



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