Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

HLA ANTIBODY (PRA)

General Information

Lab Order Codes:

PRA

Synonyms:

ALA; Donor Specific Antibody (DSA); HLA Class I/II Antibody; PRA (Panel Reactive Antibody); WBC Antibody; White Cell Antibody

CPT Codes:

No charge if the transplant was at the UM.

Test Includes:

Patient’s serum tested against purified HLA antigens to determine the presence of HLA Class I and/or HLA Class II antibody. Reflex testing, as determined by the laboratory, to determine the percent reactivity and/or specificity of the HLA antibodies present.

Logistics

Test Indications:

Useful for detection of HLA Class I and/or Class II antibodies in patient serum. High levels of HLA antibody may result in difficulty in finding a compatible organ donor.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Fairview University Labs (Fairview test code: PRA)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

Results are reported with 7 days.

Special Instructions:

Collect specimen prior to dialysis. Do not collect specimen if patient has received anti-thymocyte globulin, OKT3 or Campath recently; these drugs may case false positive results.

Specimen

Specimen Type:

Whole blood

Container:

Red-top (no gel) tube

Draw Volume:

Minimum: 15 mL blood for adult
Minimum: 9 mL blood for pediatric

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge and aliquot minimum 5 mL (3 mL for pediatric) serum.
Store refrigerated and ship at room temperature.
Protect from temperature extremes.

Patient Preparation:

None

Sample Rejection:

Gross hemolysis; immunosuppression drug interference; mislabeled or unlabeled specimens

Interpretive

Reference Range:

N/A

Critical Values:

N/A

Limitations:

N/A

Methodology:

Luminex solid phase assay

References:

Fairview-University web page (July 2014): http://labguide.fairview.org/showtest.asp?testid=3029



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