Clinics and Departments

Laboratory Services

Lab Dept:

Transfusion Services

Test Name:

PLATELET ASSOCIATED ANTIBODY

General Information

Lab Order Codes:

PLTA

Synonyms:

Platelet Associated Antibody IgG/IgM; Platelet Associated Autoantibody; Cell-Bound Platelet Autoantibody Solid Phase

CPT Codes:

86022 – Antibody identification, platelet antibodies

Test Includes:

Test results reported as positive or negative. If positive, glycoprotein specificity will be identified.

Logistics

Test Indications:

Diagnosis of: Idiopathic (autoimmune) thrombocytopenia purpura (ITP), Immune thrombocytopenia associated with systemic lupus erythematosus or other disorders associated with autoimmune phenomena.

Additional recommended testing for ITP: Platelet Antibody

Lab Testing Sections:

Transfusion Service - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 8937/CBPA)

Phone Numbers:

MIN Lab: 612-813-6820

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 - 3 days, test performed Monday - Saturday

Special Instructions:

Restricted draw times. Draw specimens Monday – Thursday only and not the day before a holiday. The patient must have a platelet count above 10,000 mm3. The specimen must not be frozen.

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube

Draw Volume:

20 mL (Minimum: Adults -10 mL/Peds – 5 mL) whole blood

Collection:

All specimens submitted must be appropriately labeled at the bedside with the time and date of collection, and the signature of the individual collecting the specimen. A completed order, either through the HIS or general requisition must accompany each specimen.

Special Processing:

Lab Staff: Do Not centrifuge. Specimen must arrive at Mayo within 48 hours of collection. Specimen should be drawn and packaged as close to shipping time as possible. Store and ship at refrigerated temperature. Verify platelet count is greater than 10,000 mm3. Forward promptly.

Patient Preparation:

The patient must be positively identified when the specimen is collected. The label on the blood specimen must correspond with the identification on the patient’s Medical Record wrist or ankle band and on the physician’s/practitioner’s orders.

Sample Rejection:

Specimen tube not properly labeled, specimen other than whole blood platelet-rich plasma collected in EDTA

Interpretive

Reference Range:

An interpretive report will be provided.

Note: A positive test, particularly to GP IIB/IIIa or Ib/IX, in the presence of thrombocytopenia (not explained by other findings) is consistent with idiopathic (autoimmune) thrombocytopenic purpura. Similarly, a positive test in a thrombocytopenic patient with systemic lupus erythematosus is consistent with an autoimmune cause. Patients who are septic may also have a positive test with reactivity against most glycoproteins. Presence of reactivity to some glycoproteins has no clearly established clinical significance.

Limitations:

Results of this test should be interpreted in light of the clinical circumstances.

Methodology:

Enzyme Linked Immunoassay (ELISA)

References:

Mayo Medical Laboratory Web Page September 2013

Updates:

4/22/2004: Test moved from American Red Cross-North Central Blood Services (NCBS), Platelet Serology Laboratory to Mayo Medical Laboratories.
9/6/2011: Added restricted draw time information.
9/24/2013: Updated collect volumes



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