Clinics and Departments

Laboratory Services

Lab Dept:

Transfusion Services

Test Name:

PLATELET ANTIGEN GENOTYPING PANEL

General Information

Lab Order Codes:

PAGT

Synonyms:

HPA1; HPA2; HPA3; HPA4; HPA5; HPA6; HPA9; HPA15; P1/ZW; Ko; Bak/Lek; Pen/Yuk; Br/Zav; Ca; Max; Gov

CPT Codes:

81400 – Molecular Pathology procedure, Level 1

Test Includes:

Panel of antigens: HPA1(P1/ZW), HPA2(Ko), HPA3(Bak/Lek), HPA4(Pen/Yuk), HPA5(Br/Zav), HPA6(Ca), HPA9(Max), HPA15(Gov)

Logistics

Test Indications:

Confirmation of antibody specificity in patient samples and identification of fetuses with incompatible platelet antigens.

Immune-mediated platelet disorders such as Neonatal Alloimmune Thrombocytopenia (NAT), Post-Transfusion Purpura (PTP) and platelet transfusion refractoriness are associated with the development of platelet-specific antibodies.

Also refer to Platelet Antibody.

Lab Testing Sections:

Transfusion Services - Sendouts

Referred to:

Mayo Medical Laboratories (Test# 90560) forward to the Blood Center of Wisconsin (Test# 5600)

Phone Numbers:

MIN Lab: 612-813-6820

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

7 – 10 days

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood, fetal/cord blood

Container:

Lavender top (EDTA) tube

Draw Volume:

5 – 10 mL (Minimum: 3 mL ) blood

1 mL fetal/cord blood

Collection:

All specimens submitted must be appropriately labeled at the point of draw with the time and date of collection, and the signature of the individual collecting the specimen. A completed order, either through the HIS or general requisition must accompany each specimen.

Special Processing:

Lab Staff: Do Not centrifuge. Specimen should remain in its original collection container. Store at room temperature. Ship at room temperature. Note: Order this test on the Mayo Access. Forward promptly.

Patient Preparation:

The patient must be positively identified when the specimen is collected. The label on the blood specimen must correspond with the identification on the patient’s Medical Record wrist or ankle band and on the physician’s/practitioner’s orders. If the patient is an outpatient, they must respond to their name and give their date of birth or be so identified by a parent or guardian.

Sample Rejection:

Gross hemolysis, sample placed in a serum separator tube, mislabeled or unlabeled specimen

Interpretive

Reference Range:

An interpretive report will be sent.

Limitations:

New variant alleles that possess polymorphisms within the region targeted by the oligonucleotide primers may not be identified with these assays.

Methodology:

PCR – Hybridization Probes

References:

Mayo Medical Laboratories Web November 2010

The Blood Center of Wisconsin November 2010

Updates:

6/16/2004: Test moved from the North Central Blood Services to Mayo Medical Laboratories forward to The Blood Center of SE Wisconsin. Note change in reference range, method, antigens available.
5/18/2005: Updated CPT coding, method & possible antigens identified
11/17/2010: Updated CPT coding, test orderable
2/12/2013: CPT update



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services