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Lab Dept:
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Urine/Stool
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Test Name:
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17-KETOSTEROIDS, URINE
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General Information
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Lab Order Codes:
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17KS
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Synonyms:
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17-KS
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CPT Codes:
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83586 – Ketosteroids, 17-; total
82570 – Creatinine, other source
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Test Includes:
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Urine 17 - Ketosteroid concentration in mg/24 hours and urine creatinine concentration in mg/dL
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Logistics
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Test Indications:
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Assess adrenal androgens. 17-Ketosteroids are elevated in Cushing’s syndrome, adrenogenital syndrome, some adrenal and gonadal tumors, pregnancy, and female pseudohermaphrodism.
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Lab Testing Sections:
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Urine/Stool - Sendouts
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Referred to:
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Esoterix, Inc (test #500230)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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3 - 7 days, test is set up 1 times per week
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Special Instructions:
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Submit an entire 24-hour urine collection. No preservative. Refrigerate specimen during collection. Deliver to laboratory as soon as possible after the collection is completed.
Note: Starting and ending times of collection are required for a timed urine collection and must be documented electronically or on the proper request form.
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Specimen
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Specimen Type:
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Urine, timed collection
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Container:
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Plastic leakproof container (No preservative). Urine GUARD® collection container is preferred for a timed urine sample.
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Draw Volume:
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Submit an entire 24-hour urine collection
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Processed Volume:
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50 mL (Minimum: 5.0 mL) aliquot of a well mixed 24-hour urine.
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Collection:
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For timed urine collections, empty the bladder, discard the voided sample, and note the start time. Collect all urine voided for the specified time period. At the end of the period, note the finishing time, add the last voided sample to the container by emptying the bladder. Bring the refrigerated container to the lab. Make sure all specimens submitted to the laboratory are properly labeled with the patient’s name, medical record number and date of birth.
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Special Processing:
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Lab Staff: Measure 24 hour urine. Add boric acid (0.5 g/100 mL) as preservative 0.5 g/100mL. Mix sample well and remove a 50 mL aliquot. Freeze aliquoted specimen in 60 mL urine container. Ship frozen. Forward promptly.
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Patient Preparation:
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N/A
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Sample Rejection:
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Specimens other than a complete 24 hour collection
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Interpretive
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Reference Range:
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Males
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1 - 4 years:
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<1.0 - 2.0 mg/24 hours
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5 - 9 years:
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<1.0 - 3.2 mg/24 hours
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10 - 12 years:
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1.0-5.0 mg/24 hours
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12 - 14 years:
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1.0 - 5.5 mg/24 hours
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14 - 16 years:
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3.0 - 13.0 mg/24 hours
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Adults:
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10.0 - 25.0 mg/24 hours
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Females
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1 - 4 years:
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<1.0 - 2.0 mg/24 hours
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5 - 9 years:
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<1.0 - 3.2 mg/24 hours
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10 - 12 years:
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1.0 - 5.0 mg/24 hours
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12 - 14 years:
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1.0 - 5.5 mg/24 hours
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14 - 16 years:
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2.5 - 8.0 mg/24 hours
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Adults:
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6.0 - 14.0 mg/24 hours
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Critical Values:
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N/A
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Limitations:
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Increased with obesity. Numerous drugs in common use cause spurious increase or decrease including carbamazepine, cephalothin, and tiaprofenic acid. Excretion of the 17-ketosteroids is variable over time. Although often used to evaluate androgenic status, this test essentially does not detect the major androgens, testosterone and dihydrotestosterone. If low androgens are anticipated, serum testosterone is the test of choice, not 17-KS.
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Methodology:
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Zimmerman Reaction after Acid Hydrolysis and Extraction
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Contraindications:
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ACTH will elevate values, as will stress.
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References:
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Esoterix, Inc Web Page www.esoterix.com
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Esoterix, Inc. “Expected Value & S.I. Unit Conversion Table” Fifth Edition
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