Clinics and Departments

Laboratory Services

Lab Dept:

Urine/Stool

Test Name:

FECAL FAT, QUANTITATIVE

General Information

Lab Order Codes:

FEF

Synonyms:

Fat, Feces; Stool Fat; Lipids Stool

CPT Codes:

82710 – Fat or lipids, feces; quantitative

Test Includes:

Fat concentration in g fat/24 hr or % fat reported in a random collection.

Logistics

Test Indications:

Diagnosing fat malabsorption due to pancreatic insufficiency or intestinal disorders and for monitoring effectiveness of enzyme supplementation in certain malabsorption disorders.

Lab Testing Sections:

Urine/Stool - Sendouts

Referred to:

Mayo Medical Laboratories (Test: FATF/8310)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 - 5 days, test set up Monday – Friday

Special Instructions:

Length of collection period is required on request form for processing. Obtain special collection containers from the lab. Random collections require at least 5 g of stool.

The use of a charcoal marker is not recommended. If charcoal is used, please notify the laboratory.

See Patient Preparation

Note: Other desired stool testing may not be able to be shared with a specimen collected for Fecal Fat testing. Please contact the laboratory for information on multiple tests on stool specimens or see Patient Preparation.

Specimen

Specimen Type:

Stool, 48 or 72 hour collection preferred (24 hour accepted). Random stools can be tested if at least 5 grams of stool has been collected.

Container:

Special containers fecal fat collection containers (Mayo Supply T291).

Draw Volume:

Entire stool collection (Minimum: 5 g stool)

Processed Volume:

Specimen will be processed at the reference lab facility. A minimum of 5 grams of feces is required for testing.

Collection:

At the time the container is given to the patient, please:

--Fill in the patient name on the specimen container label.

Review the test to be done and specimen requirements with the patient.

--Collection duration

--Diet requirements

--Collection and storage of the specimen until it is returned to you

--Four MML containers should be provided for a timed collection

--Provide patient with information on how to obtain additional containers should that be necessary.

--Instruct patient to not fill any container more than 3/4 full (to the indicated line on the label).

At the time the patient returns the container, complete the following information on the label:

Duration of collection should have the appropriate box checked. If timed duration is other than those listed, please list it on the line provided.

Indicate if the entire collection is contained in one container or in multiple containers. Indicate total number of containers sent.

Special Processing:

Label each container with the MML Control Number sticker from the appropriate request form. Send entire collection frozen in containers supplied by MML.

Patient Preparation:

1. For 3 days prior to and during the collection period:

● Patient should be on a fat-controlled diet (100 - 150 g fat per day)

● No laxatives (particularly mineral oil and castor oil)

● No synthetic fat substitutes (eg, Olestra) or fat blocking nutritional supplements

2. Barium interferes with this test procedure; a waiting period of 48 hours before stool collection analysis is recommended.

3. Label all containers, ie, 1 of 2, 2 of 2, etc. and send all containers.

4. A separate order and collection should take place if stool calcium, chloride, magnesium, osmolality, pH, potassium, sodium, zinc, or any microbiology testing is desired.

5. The use of diaper rash ointments will falsely elevate test results. Discontinue use during the collection period.

Sample Rejection:

Specimens other than feces, mislabeled or unlabeled specimens

Interpretive

Reference Range:

Timed Collections

0 – 17 years:

Not established

>or = 18 years:

2 - 7 g fat/24 hours

Random Collections

All ages:

0 – 19 % fat

Excretion of >7 grams fat/24 hours, when on a diet of 100 g to 150 g of fat, is suggestive of a malabsorption defect.

Abnormal values should be confirmed by submission of a time collection.

Test values for timed fecal fat collections will be reported in terms of g/24 hours; the duration of the collection may be 24, 48, 72 or 96 hours. Test values for random fecal fat collections will be reported in terms of % fat.

Critical Values:

N/A

Limitations:

Not useful for differentiating among pancreatic diseases. Proper patient preparation is critical. Failure to adhere to a fat-controlled diet or to exclude other oil/oil substitutes from the diet may make interpretation difficult. Barium interferes with this test procedure. A waiting period of 48 hours before stool collection analysis is recommended.

The use of a charcoal marker is not recommended. If charcoal is used, please notify the laboratory.

Methodology:

Nuclear magnetic resonance (NMR) spectroscopy

References:

Mayo Medical Laboratories May 2013

Updates:

6/29/2004: Specimen method changed from gravimetric to nuclear magnetic resonance.
12/20/2007: Added information regarding collection of random specimens.
8/12/2010: Updated reference ranges for age.



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