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Lab Dept:
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Urine/Stool
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Test Name:
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TRYPSIN, STOOL
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General Information
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Lab Order Codes:
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TRYPS
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Synonyms:
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Stool Trypsin; Feces for Trypsin
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CPT Codes:
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84488 – Trypsin; feces, qualitative
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Test Includes:
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Evaluation of trypsin activity in stool.
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Logistics
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Lab Testing Sections:
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Microbiology - Sendouts
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Referred to:
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Mayo Medical Laboratories forward to ARUP (MML Test: FFTRF)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-5866
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651-220-6555
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 3 days
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Special Instructions:
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Requisition must state specific date/time of collection. The stool for this test cannot be shared with other tests. If other testing is required, collect separate stool samples.
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Specimen
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Specimen Type:
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Fresh random stool
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Container:
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Plastic, leakproof container
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Volume:
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5 grams random stool
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Collection:
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● Collect stool in a clean, dry bedpan or on a newspaper over the toilet. Transfer specimen to a plastic, leakproof container. Do not overfill or contaminate the outside of the container.
● Pediatric patients with severe diarrhea may use a U-bag collection system. Place the bag over the anal area in an attempt to retrieve the specimen before it soaks into the diaper. The diaper can also be reversed with the plastic side toward the skin to prevent the specimen from soaking into the diaper. Transfer specimen into a plastic, leakproof container.
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Transport/Storage:
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Specimen must arrive within 1 hour of collection. Do not send specimen through the pneumatic tube. Freeze specimens immediately. No preservative. Send frozen on dry ice in a plastic container.
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Patient Preparation:
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Barium procedures and laxatives should be avoided for 1 week prior to collection of the specimen.
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Sample Rejection:
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No diapers accepted. Specimens exceeding 1 hour transit time; improperly labeled specimen; specimen contaminated with urine and/or water; leaking container; insufficient volume; specimen containing interfering substances such as castor oil, bismuth, Metamucil®, barium, Vaseline®, or other cream contaminants. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded. Mislabeled or unlabeled specimens. Specimens affected by freeze/thaw cycles.
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Interpretive
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Reference Range:
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Greater than or equal to 1:96
Note: A fecal titer greater than or equal to 1:96 is considered normal. Fecal trypsin is measured by serially diluting stool in buffer and identifying the greatest titer at which x-ray film is ceared of its gelatin emulsion.
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Limitations:
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Frozen specimens are only stable for 1 week.
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Methodology:
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Digestion of gelatin emulsion on X-ray film using serial dilutions of buffer and stool.
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References:
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Mayo Medical Laboratories August 2012
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Updates:
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9/1/2010: Testing no longer done in house. Now referred to Mayo and forwarded to ARUP.
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