Clinics and Departments

Laboratory Services

Lab Dept:

Urine/Stool

Test Name:

STOOL PMN’S

General Information

Lab Order Codes:

PMNS

Synonyms:

Smear for Fecal Leukocytes; Stool WBC”s; Stool PMN’s; Fecal Leukocyte Smear

CPT Codes:

87205 – Smear, primary source with interpretation; Gram or Giemsa stain for bacteria, fungi or cell types

Test Includes:

Smear and quantitation of leukocytes in stool.

Logistics

Test Indications:

Useful for conditions associated with increased fecal leukocytes including antibiotic-associated colitis, ulcerative colitis, shigellosis, salmonellosis, Campylobacter, Yersinia and amoebiasis

Lab Testing Sections:

Microbiology

Phone Numbers:

MIN Lab: 612-813-5866

STP Lab: 651-220-6555

Test Availability:

Daily, 24 hours

Turnaround Time:

24 hours

Special Instructions:

Requisition must state specific date/time of collection.

Specimen

Specimen Type:

Fresh diarrheal stool, especially with blood or mucus

Container:

Plastic, leakproof container

Draw Volume:

1 gram stool

Collection:

1. Collect fresh, diarrheal stool in a clean, dry bedpan or on a newspaper over the toilet and transfer to a plastic, leakproof container.

2. Those portions of stool containing blood and mucous are especially significant and should be transferred into the container.

3. Pediatric patients with severe diarrhea may use a U-bag collection system. Place the bag over the anal area in an attempt to retrieve the specimen before it soaks into the diaper. The diaper can also be reversed with the plastic side toward the skin to prevent the specimen from soaking into the diaper. Transfer specimen into a plastic, leakproof container.

Transport/Storage:

Specimen must arrive in laboratory within 1 hour of collection. Do not send specimen through the pneumatic tube system.

Sample Rejection:

No diapers accepted. Improperly labeled specimen; specimen contaminated with urine and/or water; leaking container; insufficient volume; specimen containing interfering substances such as castor oil, bismuth, Metamucil®, barium, Vaseline®, or other cream contaminants. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.

Interpretive

Reference Range:

Occasional PMN’s present

Limitations:

False-negative results may occur if patient is on antibiotics.

Methodology:

Gram stain

References:

Cook, JH, and M Pezzlo (1992). Specimen receipt and accessioning. Section 1. Aerobic bacteriology, 1.2.1-4. In HD Isenberg (ed) Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington DC

Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC

Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104

Updates:

1/6/2014: Turnaround time previously listed as 4 hours.



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