Lab Dept:

Urine/Stool

Test Name:

17-HYDROXYPROGESTERONE, URINE

General Information

Lab Order Codes:

17HU

Synonyms:

17-Hydroxy Progesterone, Urine; 17-OH Progesterone, Urine

CPT Codes:

83498 – Hydroxyprogestone, 17-d

Test Includes:

Urine 17-Hydroxy Progesterone is measured by radioimmunoassay following extraction of specimens.

Logistics

Test Indications:

17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone. It is converted enzymatically to Androstenedione and 11-Deoxycortisol. It is produced in both the gonads and adrenal glands. It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol. This assay measures the total of the conjugated and unconjugated forms. It is stimulated by ACTH and supressed by Dexamethasone. Levels of urine 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases. 17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia. Urine levels are frequently elevated in patients with idiopathic hirsutism.

Lab Testing Sections:

Urine/Stool - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test# 90170) and forwarded to Inter Science Institute

Phone Numbers:

Minneapolis:

Saint Paul:

612-813-6280

651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 – 7 days; performed Monday - Friday

Special Instructions:

Refrigerate or keep specimen on ice during 24 hour collection. There is no preservative. See Patient Preparation.

Specimen

Specimen Type:

Urine, 24 hour collection

Container:

Plastic, leak-proof urine collection container

Draw Volume:

Entire collection

Processed Volume:

5.0 mL of urine from a well-mixed 24 hour collection

Collection:

Routine 24 hour urine collection, refrigerate or keep on ice

Special Processing:

Lab Staff: Mix 24 hour specimen well. Measure and record total urine volume. Remove 5.0 mL aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

Patient should not be on any Corticosteriod, ACTH, steroid, estrogen, or gonadotropin medication, if possible, for at least 48 hours prior to start of urine collection.

Sample Rejection:

Specimens other than urine, mislabeled specimens or unlabeled specimens

Interpretive

Reference Range:

17-Hydroxyprogesterone:

Age

Range

3 days – 1 year:

Up to 50 ng/24 hours

1 – 8 years:

Up to 300 ng/24 hours

Adult Males:

Up to 2.0 ug/24 hours

Adult Females:

Up to 4.5 ug/24 hours

Critical Values:

N/A

Limitations:

N/A

Methodology:

Radioimmunoassay (RIA) following extraction of specimens

References:

Mayo Medical Laboratory Web Page March 2010

Inter Science Institute March 2010