Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

HEPATITIS Bs ANTIGEN (HBsAg) NEUTRALIZATION (CONFIRMATION)

General Information

Lab Order Codes:

HBNE

Synonyms:

Hepatitis B Surface Antigen Neutralization

CPT Codes:

87341 – HbsAg neutralization (confirmation)

Test Includes:

Neutralization of Hepatitis B Surface Antigen. This test is not orderable separately. It will automatically be performed as a reflex on all positive HbsAg tests.

Logistics

Test Indications:

Orderable only by laboratory staff based on positive result.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (Test# 84396)

Phone Numbers:

Minneapolis:

Saint Paul:

 

612-813-6280

651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days, test set up daily.

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood

Container:

Additional specimen is not required. See Hepatitis Bs Antigen (HbsAg) Reflex.

Draw Volume:

Additional specimen is not required. See Hepatitis Bs Antigen (HbsAg) Reflex.

Processed Volume:

Additional specimen is not required. See Hepatitis Bs Antigen (HbsAg) Reflex.

Collection:

N/A

Special Processing:

N/A

Patient Preparation:

None

Sample Rejection:

N/A

Interpretive

Reference Range:

Negative (reported as positive or negative)

Critical Values:

N/A

Limitations:

N/A

Methodology:

The HbsAg confirmatory assay uses the principle of specific antibody neutralization to confirm the presence of HbsAg. The confirmatory reagent (human antibody to HbsAg) is incubated with the specimen in solution. If HbsAg is present in the specimen it will be bound by the confirmatory reagent. The neutralized HbsAg is subsequently blocked from binding to the antibody-coated bead. This results in a reduction of signal when compared to the non-neutralized specimen in which the negative control is used in place of the confirmatory reagent. By definition, a specimen is confirmed as positive if the reduction in signal of the neutralized specimen is at least 50% and the non-neutralized control generates a signal greater than or equal to the assay cut-off. (Package insert: Antibody to Hepatitis B Surface Antigen [Human] – HBsAg Confirmatory Assay. Abbott Laboratories, Diagnostics Division, Abbott Park, IL)

Contraindications:

N/A

References:

Mayo Medical Laboratories Web Page June 2004 http://www.mayomedicallaboratories.com/

Updates:

4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories.



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