Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



Complement, Total; Complement, Total Hemolytic

CPT Codes:

86162 – Complement; total hemolytic (CH50)

Test Includes:

Total complement level reported in U/mL.


Test Indications:

Useful for detection of individuals with on ongoing immune process or congenital complement deficiencies.

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 8167/COM)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 2 days, test set up Monday - Saturday

Special Instructions:

See Collection. See Patient Preparation.


Specimen Type:

Whole blood


Red top tube (plain, no gel)

Draw Volume:

3 mL (Minimum: 1.5 mL) blood

Processed Volume:

1 mL (Minimum: 0.5 mL) serum


Routine venipuncture, immediately place specimen on wet ice and transport to lab for processing.

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

Fasting specimens preferred.

Sample Rejection:

Warm specimens, mislabeled or unlabeled specimens


Reference Range:

≥16 years:

30 – 75 U/mL

Interpretation: Low levels of complement (total hemolytic complement: CH50) may occur during infections, disease exacerbation in patients with systemic lupus erythematosus, and in patients with immune complex diseases such as glomerulonephritis.

Undetectable levels suggest the possibility of a complement component deficiency. Individual complement component assays are useful to identify the specific deficiency.

Critical Values:



Because this is a functional assay, the results are dependant on appropriate specimen transport and storage.


Automated Liposome Lysis Assay


Mayo Medical Laboratories July 2013

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