Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



Mitogen Studies; Blastogenesis Mitogens; Blastogenesis Antigens; Lymphocyte Transformation

CPT Codes:

86353 x 2 – Lymphocyte transformation, mitogen or antigen induced blastogenesis

Test Includes:

Lymphocyte Proliferation, Antigens and Mitogens. An interpretive report of findings will be provided. For more information see individual test listings.


Test Indications:

Evaluating patients suspected of having diminished cellular immune function; evaluating patients with primary and secondary immunodeficiency diseases that affect T lymphocytes; Evaluating functional T-cell recovery post-hematopoietic stem cell transplant or immunosuppressive therapy for solid-organ or in other clinical contexts.

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 60593/LPMAF)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

8 - 11 days, test set up Monday - Friday

Special Instructions:

Specimen must arrive within 24 hours of draw. Send specimen Monday - Thursday only. See Collection for important information. Obtain special tube from laboratory. For serial monitoring, it is recommended that the sample be collected at the same time of day per collection.


Specimen Type:

Whole blood


Green top (Na Heparin) tube. Lithium heparin is not acceptable.

Draw Volume:

Draw volume varies by age. Reference table below:

Patient Age

Requested Volume

Minimum Volume

<3 months

3 mL

1 mL

3 – 24 months

5 mL

3 mL

25 months – 5 years

6 mL

5 mL

6 - 18 years

8 mL

5 mL

>18 years

30 mL

20 mL

Processed Volume:

Same as Draw Volume


Routine venipuncture, send specimen to the laboratory immediately after collection. Note: Specimens must be filled by needle through the stopper to maintain a closed system. DO NOT fill tube by removing the stopper.

Special Processing:

Lab Staff: Do Not centrifuge. Send in original collection tube. Keep at room temperature. Forward promptly.

Patient Preparation:


Sample Rejection:

Specimens other than whole blood; anticoagulants other than sodium heparin; frozen specimens; gross hemolysis; gross lipemia; mislabeled or unlabeled specimens


Reference Range:

See individual test listings, Lymphocyte Proliferation, Antigens and Mitogens. A narrative report is provided with each result.

Critical Values:



See individual test listings, Lymphocyte Proliferation, Antigens and Mitogens


Flow cytometry


Mayo Medical Laboratories Web Page January 2011


1/18/2011: Method change, reference range change, draw volume update.

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