Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



Human Immunodeficiency Virus Types 1 and 2 (combined) Antibodies; Anti-HIV-1,2; Anti-HIV

CPT Codes:

87389 – HIV-1 antigen, with HIV1/2 antibodies, single result
86701 – HIV-1 antibody (if appropriate)
86702 – HIV antibody-type 2 (if appropriate)

Test Includes:

HIV-1,2 Ag/Antibody reported as negative or reactive. If HIV-1,2 ag/antibody is reactive, then HIV-1,2 antibody differentiation is performed (by rapid immunographic method) at an additional charge.


Test Indications:

Screening and confirmation of HIV-1 and HIV-2 infection.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (Test# HIVCO)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 2 days, tests performed Monday - Saturday. Confirmatory testing may increase turnaround times.

Special Instructions:

This test is not offered as a screening or confirmatory test for blood donor specimens. See Patient HIV Testing Algorithm ( ) for more information.


Specimen Type:



Red, marble or gold (SST)

Draw Volume:

3 mL (Minimum: 1.5 mL) blood

Processed Volume:

1 mL (Minimum 0.5 mL) serum


Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen within 24 hours, remove serum/plasma aliquot into screw-capped round bottom plastic vial. Store and ship at refrigerated temperatures. Forward promptly.

Patient Preparation:


Sample Rejection:

Specimens other than serum/plasma as indicated; lithium heparin samples are unacceptable; mislabeled or unlabeled specimens; gross hemolysis; gross lipemia; grossly icteric


Reference Range:

Negative (reported as negative or reactive)

Additional testing at an additional charge will be performed if the specimen is reactive. Refer to Test Includes:

Critical Values:



Reactive results of this assay does not differentiate among reactivity with HIV-1 p21 antigen, HIV-1 antibody, and HIV-2 antibody.

A reactive screening test result is not diagnostic for HIV infection and should be considered preliminary. The positive predictive value of a reactive screening test result is highly dependent o the prevalence of HIV infection in the population tested. The lower the prevalence of HIV infection, the lower the positive predictive value and higher the false-positive rate of the test. Diagnosis of HIV infection must be based on positive results of the supplemental or confirmatory serologic or molecular tests.


HIV-1, Ag/Ab Screen and HIV-2 Aby: Chemoluminescenct particle assay
HIV-1 Differentiation: Rapid Immunographic method
HIV-2 Differentiation: Rapid Immunographic method


Mayo Medical Laboratories Web Page June 2016


4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories. Note: Test now reflexes to supplemental/confirmatory testing (with additional charges) when indicated by reactive findings.
11/18/2008: Method change, previously listed as Enzyme Immunoassay
3/13/2012: EDTA no longer appropriate tube type.
3/1/2013: HIV-2 confirmation now preformed by immunoassay at Mayo.
5/19/2014: Method change previously listed as HIV1/2 Aby Scn by Chemoluminescence (EIA). Now also screen for HIV-1 antigen. CPT
code change for screening test. Previously listed as 86709.


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