Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



Leptospira Agglutination, Serum; Weil’s disease; Leptospirosis Antibody

CPT Codes:

86720 – Antibody; Leptospira

Test Includes:

Leptospira Antibody reported as negative or as a titer.


Test Indications:

As an aid in the diagnosis of leptosprirosis.

Known exposure to contaminated water. Usually abrupt onset with fever; may occur in 2 phases. Phase 1 has fever, chills, headache, muscle aches, vomiting or diarrhea. The patient may recover for a time but will become ill again. If a second phase occurs, it is more severe. The person may have kidney or liver failure or meningitis. This phase is also called Weil’s disease. Incubation range is 2 days–4 weeks.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 9697/LEPTO)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 5 days, test performed Monday, Wednesday and Friday

Special Instructions:



Specimen Type:



Red top tube

Draw Volume:

1.5 mL (Minimum: 0.5 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.15 mL) serum


Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot. Store and ship at refrigerated temperatures. Forward promptly.

Patient Preparation:


Sample Rejection:

Specimens other than serum; gross hemolysis, gross lipemia; mislabeled or unlabeled specimens


Reference Range:


Interpretation: Both IgM and IgG classes of antibody are produced in response to any of 16 leptospira serovars that produce infection. IgM antibody is first detectable within 1 to 2 weeks after onset of illness and peaks at 2 to 4 weeks.

Titers of 1:50 are considered borderline and follow-up specimens should be drawn for isolation of live leptospires (ie, culture) and repeat serology.

Titers >or =1:100 represent recent or active infection.

The overall sensitivity of the assay is 100% and the specificity is 97% when compared to the Centers for Disease Control and Prevention reagents.

Critical Values:



Serologic results must be correlated with the clinical picture. Sera drawn too close to onset of symptoms may precede the initial IgM antibody response. Acute titers may be delayed or substantially decreased by early and intensive antibiotic treatment. The assay detects antibody to the genus Leptospira but will not determine which serovar is associated with infection.


Indirect Hemagglutination


Mayo Medical Laboratories July 2013


3/25/2004: Test moved from the Minnesota Department of Health to Mayo Medical Laboratories.
4/24/2008: Draw volumes temporarily increased for forward to ARUP.
2/17/2011: Testing performed internally at MML. Reference range and volume update.

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