Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



MS Profile

CPT Codes:

82040 - Albumin, serum
82042 - Albumin, spinal fluid
82784 x2 - IgG, serum and spinal fluid
83916 - Oligoclonal band, spinal fluid
83916 - Oligoclonal band, serum, if appropriate

Test Includes:

CSF bands, Serum bands, CSF Oligoclonal bands interpretation, CSF IgG Index, Serum IgG. Testing requires both serum and cerebral spinal fluid.


Test Indications:

Diagnosing MS, especially helpful in patients with equivocal clinical or radiological findings.

Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disease characterized by visual, motor, and sensory disturbances. The diagnosis of MS is dependent on clinical, radiological and laboratory findings. The detection of increased intrathecal immunoglobulin (Ig) synthesis is the basis for current diagnostic laboratory tests for MS.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test#:MSP2)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

3 days

Special Instructions:

Both blood and spinal fluid need to be collected.


Specimen Type:

Blood and spinal fluid (CSF)


Blood: Red top tube
Spinal Fluid: CSF conical tube

Draw Volume:

Blood: 3 mL (Minimum: 1.5 mL)
Spinal Fluid: 1 mL (Minimum: 0.5 mL)

Processed Volume:

Serum: 1 mL (MInimum: 0.5 mL)
Spinal Fluid: 1 mL (Minimum: 0.5 mL)


Blood: Routine venipuncture
Spinal Fluid: Routine CSF collection

Special Processing:

Lab Staff:
Blood: Centrifuge specimen and aliquot into a round bottom, screw-capped plastic vial. Label specimen as serum. Store and ship at refrigerated temperatures.

Spinal Fluid: Aliquot spinal fluid into a round bottom, screw-capped plastic vial. Label as spinal fluid. Store and ship at refrigerated temperatures.

Patient Preparation:


Sample Rejection:

Mislabeled or unlabeled specimens


Reference Range:

Test Name:


Oligoclonal bands

<4 bands

CSF IgG Index



≤8.1 mg/dL

CSF albumin

≤27.0 mg/dL

Serum IgG

600 - 1500 mg/dL

Serum albumin

3200 - 4800 mg/dL

CSF IgG/albumin


Serum IgG/albumin


CSF IgG synthesis rate

≤12 mg/24 h

Interpretation: Oligoclonal bands ≥4 CSF -specific bands are consistent with MS. CSF IgG index: >0.85 is consistent with MS. Abnormal CSF IgG indexes and oligoclonal band patterns have been reported in 70-80% of MS patients. If both tests are performed, at least 1 of the results has been reported to be positive in >90% of MS patients. A newer methodology for oligoclonal bands, isoelectric focusing (IEF) has been reported to be more sensitive (90-95%).

Critical Values:



Increased intrathecal Ig synthesis may occur in other inflammatory central nervous system (CNS) diseases and therefore, these assays are not specific for MS. The presence of oligoclonal bands or elevated CSF Ig index is unrelated to disease activity.


Isoelectric focusing with IgG Immunoblot Detection; Nephelometry


Mayo Medical Laboratories July 2015

  Lab Test Directory
    Microbiology/ Virology
    Other Fluids
    Transfusion Services

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services