Clinics and Departments

Laboratory Services

Lab Dept:


Test Name:


General Information

Lab Order Codes:



17-Hydroxy Progesterone, Urine; 17-OH Progesterone, Urine

CPT Codes:

83498 – Hydroxyprogestone, 17-d

Test Includes:

Urine 17-Hydroxy Progesterone is measured by radioimmunoassay following extraction of specimens.


Test Indications:

17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone. It is converted enzymatically to Androstenedione and 11-Deoxycortisol. It is produced in both the gonads and adrenal glands. It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol. This assay measures the total of the conjugated and unconjugated forms. It is stimulated by ACTH and supressed by Dexamethasone. Levels of urine 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases. 17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia. Urine levels are frequently elevated in patients with idiopathic hirsutism.

Lab Testing Sections:

Urine/Stool - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 90170/F17HP) and forwarded to Inter Science Institute

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

5 – 9 days; performed Monday - Friday

Special Instructions:

Refrigerate or keep specimen on ice during 24 hour collection. There is no preservative. See Patient Preparation.


Specimen Type:

Urine, 24 hour collection


Plastic, leak-proof urine collection container

Draw Volume:

Entire collection

Processed Volume:

10 mL (Minimum: 5 mL) urine from a well-mixed 24 hour collection


Routine 24 hour urine collection, refrigerate or keep on ice

Special Processing:

Lab Staff: Mix 24 hour specimen well. Measure and record total urine volume. Remove aliquot into a screw-capped round bottom plastic vial. Provide total 24 hour volume. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

Patient should not be on any Corticosteriod, ACTH, steroid, estrogen, or gonadotropin medication, if possible, for at least 48 hours prior to start of urine collection.

Sample Rejection:

Specimens other than urine, mislabeled specimens or unlabeled specimens


Reference Range:




3 days – 1 year:

Up to 50 ng/24 hours

1 – 8 years:

Up to 300 ng/24 hours

Adult Males:

Up to 2.0 ug/24 hours

Adult Females:

Up to 4.5 ug/24 hours

Critical Values:





Radioimmunoassay (RIA) following extraction of specimens


Mayo Medical Laboratory Web Page August 2013

Inter Science Institute August 2013

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