Lab Dept:


Test Name:


General Information

Lab Order Codes:



AHF; AHG; Antihemophiliac Factor; FVIII; VIIIC; Factor VIII Activity

CPT Codes:

85240 - Clotting; factor VIII (AHG), one stage

Test Includes:

Factor 8 level reported as a %.


Test Indications:

Useful for the detection of a single factor congenital deficiency for Hemophilia A or von Willebrand’s disease or an acquired deficiency due to liver disease or DIC.

Lab Testing Sections:


Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

4 hours

Special Instructions:

Patient should not be receiving heparin. If so, this should be noted on the request form. Heparin therapy will affect certain coagulation factors or assays, preclude their performance, or cause spurious results. Indicate when specimen is drawn from a line or a heparin lock. Deliver immediately to the laboratory.


Specimen Type:

Whole blood


Light Blue top (Buffered Na Citrate 3.2%) tube

Draw Volume:

2.7 mL blood

Processed Volume:

0.9 mL plasma


● A clean venipuncture is essential, avoid foaming. ● Entire sample must be collected with single collection, pooling of sample is unacceptable.
● Capillary collection is unacceptable.
● Patient’s with a hematocrit level >55% must have a special tube made to adjust for the hematocrit; contact lab for a special tube.
● Mix thoroughly by gentle inversion. Deliver immediately to the laboratory at room temperature via courier or pneumatic tube.

Off campus collections:
● Must be tested within 4 hours. ● Do not refrigerate.
● If not received in our lab within 4 hours of collection, sample must be centrifuged and *platelet-poor plasma removed from cells and transferred to an aliquot tube being careful not to disturb the cell layer. Centrifuge the plasma a second time and transfer into a clean aliquot tube being careful not to include any residual platelets on the bottom of the tube. Freeze at -20°C and deliver to the lab on dry ice within 2 weeks.
*Validation of your lab’s centrifuge for platelet poor plasma is required.

Special Processing:

Lab Staff: Spin sample collected in blue top tube(s) for 5 minutes on the Stat Spin centrifuge, remove plasma and transfer to a 4 mL BCS sample cup(s), spin remaining plasma again for 5 minutes in the Stat Spin Centrifuge. Transfer plasma to new BCS sample cup(s) for analysis (as specifically stated in each procedure) leaving approximately 200 mcL in the bottom of the original cup to discard.

Patient Preparation:

Avoid heparin therapy for two days prior to the test.

Sample Rejection:

Improper tube; clotted samples; underfilled tube; mislabeled or unlabeled specimens


Reference Range:



0 – 1 day

61 – 139%

2 – 5 days

55 – 121%

6 – 30 days

58 – 124%

31 – 90 days

56 – 102%

91 – 180 days

55 – 91%

6 months – 5 years

59 – 142%

6 – 10 years

55 – 120%

11 – 16 years

53 – 131%

>16 years

50 – 150%

Critical Values:



Interpretation of the results may be limited if patient is receiving anticoagulant therapy or if test is done more then 2 hours after collection. Results will be increased in patients taking oral contraceptives.


APTT clotting time correction of factor 8 deficient plasma. Patient dilutions are compared to a known set of standard dilutions and a percentage is determined.


Patient on anticoagulant therapy.


Andrew M et al (1987) Development of the Human Coagulation System in the Full-Term Infant, Blood 70:165-72

Andrew M et al (1988) Development of the Human Coagulation System in the Healthy Premature Infant 72:1651-57

Andrew M et al (1992) Development of the Human Coagulation System During Early Childhood 80:1998-2005


5/24/2010: Tubing of patient specimens is no longer prohibited.
12/15/2010: Updated processing information.
9/15/2014: Added Off Campus collection info.