Lab Dept:


Test Name:

Hemoglobin s and F monitoring

General Information

Lab Order Codes:



Hbg S and F Quantitation for Therapeutic Monitoring

CPT Codes:

83021 – Hemoglobin fractionation and quantitation; chromatography 85660 – Sickling of red blood cells, reduction (if appropriate)

Testing includes:


Hemoglobin S and F measurement by Cation Exchange/High-Performance Liquid Chromatography (HPLC). If hemoglobin S is detected, hemoglobin S screen will be performed when appropriate at an additional charge.


Test Indications:

Monitoring patients with sickling disorders who have received hydroxyurea or transfusion therapy. This test is not intended for diagnostic purposes; it is assumed the patient’s diagnosis is established. If the patient has never been studied, hemoglobin electrophoresis is necessary.

Lab Testing Sections:

Hematology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: SFMON)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 days, performed Monday - Saturday

Special Instructions:

This test is not intended for diagnostic purposes. See Test Indications.


Specimen Type:

Whole blood


Lavender (EDTA) top tube

Draw Volume:

6 mL (Minimum: 1 mL) blood

Processed Volume:

Same as Draw Volume.


Routine venipuncture


Special Processing:

Lab Staff: Do Not centrifuge. Specimen should remain in original collection container. Do not freeze. Send refrigerated.

Patient Preparation:


Sample Rejection:

Testing cannot be performed on clotted samples; frozen specimens; mislabeled or unlabeled specimens; gross hemolysis


Reference Range:

Hgb Fraction



Hgb F

1 – 30 days:

22.8 – 92.0%

1 – 2 months:

7.6 – 89.8%

3 – 5 months:

1.6 – 42.2%

6 – 8 months:

0.0  – 16.7%

9 – 12 months:

0.0  – 10.5%

13 – 17 months:

0.0  – 7.9%

18 – 23 months:

0.0  – 6.3%

>or = 24 months:

0.0  – 0.9%

Hgb S

All ages:


Hgb S, Screen

All ages:


Critical Values:



This test is not intended for diagnostic purposes. See Test Indications.

This assay will not discriminate hemoglobin S (Hb S) trait from homozygous sickle cell disease (S/S), nor any of the following combinations: S/C, S/D, S/G, S/E, S/beta-thalassemia, S/O-Arab, and C-Georgetown trait. The presence of HbS will be confirmed, but any additional Hb variants will not be definitively identified.


Hemoglobin F/S: Cation Exchange/HPLC
Hemoglobin Electrophoresis: Capillary Electrophoresis
Hemoglogin S: Hemoglobin S Solubility


Mayo Medical Laboratories Lab Web Page August 2015