Lab Dept:

Anatomic Pathology

Test Name:


General Information

Lab Order Codes:

Orders are communicated via the Surgical Pathology request form


Ciliary biopsy; Cilia motility; Immotile cilia; Cilia B Frequency

CPT Codes:

88305 – Level 4, surgical pathology, gross and microscopic exam 88329 – Pathology consult during surgery
88348 – Electron microscopy, each sampled site

Test Includes:

Electron microscopy (EM) of Cilia biopsy


Test Indications:

Useful for evaluating cilia for motility and ultrastructure in cases of chronic upper respiratory infection and/or suspected immotile cilia syndrome or Kartegener Syndrome.

Lab Testing Sections:

Anatomic Pathology – Sendouts

Referred to:

Mayo Medical Laboratories

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Monday - Thursday

Turnaround Time:

7 - 21 days

Special Instructions:

Obtain the biopsy from the upper airways or the carina. The mucosa over the adenoids is more likely to yield an interpretable sample of cilia; turbinate biopsies frequently show inflammation-associated loss of ciliated epithelium.


Specimen Type:



Biopsy for electron microscopy: obtain container with fixative from laboratory.

Draw Volume:

See Collection

Processed Volume:

See Collection


Specimen for direct observation of motility: transport fresh to lab immediately in lactate ringers. Solution can be obtained from the Histology Lab.

Specimen for electron microscopy: place in fixative immediately upon biopsy.

Trump's fixative (4% formalin:  1% glutaraldehyde) (Supply T130) is optimal. If no tissues fixed primarily in glutaraldehyde are available, shavings can be taken from the surface of tissues in formalin, where fixation was most rapidly achieved.


Special Processing:


Patient Preparation:

Physician preference

Sample Rejection:

Improper specimen, unlabelled or mislabeled specimen


Reference Range:

Interpretive report (final reports will be scanned into the HIS and copies distributed to the ordering MD and surgeon)

Critical Values:



Artifactual loss of cilia or secondary loss of cilia due to chronic inflammatory changes, insufficient cilia numbers for definite diagnosis by electron microscopy.


Direct semi-phase-contrast microscopy, routine light microscopy, transmission electron microscopy


Pathology Department, Children’s Hospitals and Clinics


Mayo Medical Laboratories, February 2012