Lab Dept:


Test Name:

Celiac Disease Panel Reflex

General Information

Lab Order Codes:



Celiac Disease Panel

CPT Codes:

83516 x4 – Immunoassay for analyte other than infectious agent antibody or infectious agent antigen, qualitative or semiquantitative; multiple step method
82784 – Total IgA (if appropriate)

Test Includes:

Anti-Deamidated Gliadin IgA Specific, Anti-Deamidated Gliadin IgG Specific, Anti-Tissue Transglutaminase IgA Specific, Anti-Tissue Transglutaminase IgG Specific; TTGA; TTGG

Reflex: Total IgA will be performed at an additional charge when tTG-IgA is negative, and tTG-IgG is positive, or when the instrument flags a low IgA


Test Indications:

Useful for differential diagnosis of intestinal disorders such as celiac disease and Crohn’s disease. May be useful for monitoring a gluten-free diet.

Lab Testing Sections:

Chemistry – Minneapolis campus only

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 4 days; performed Monday - Friday

Special Instructions:

Endomysial Antibody is orderable separately and referred to Mayo Medical Laboratories for testing.


Specimen Type:



Red top tube

Draw Volume:

1.5 mL (Minimum: 0.9 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.3 mL) serum


Routine venipuncture


Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot and transfer into a screw-top plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.

Patient Preparation:


Sample Rejection:

Mislabeled or unlabeled specimen


Reference Range:

Test Name
(all ages)

(Elia U/mL)

(Elia U/mL)

(Elia U/mL)

Tissue transglutaminase IgA (TTGA)


7 - 10


Tissue transglutaminase IgG (TTGG)


7 - 10


Deamidated Gliadin antibody IgA (GGP)


7 - 10


Deamidated Gliadin antibody IgG (AGP)




Reflexive Total IgA (mg/dL)

Please refer to IgA (Immunoglobulin A) for reference ranges.

TTGA >10 Elia U/mL: Suggestive of Celiac disease
TTGA and TTGG both >10 U/mL: Suggestive of Celiac disease
AGP >10 Elia U/mL or AGP and GLG >10 Elia U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy

Critical Values:





Fluorenzyme immunoassay


Phadia AB Elia Directions for Use (August 2010)

CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition

Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition


1/11/2007: CPT 2007 update
10/9/2007: Gliadin IgG and IgA updated to deamidated ELISA tests.
10/14/2008: tTG reference range/units update previously listed as <20 Units.
11/2/2009: Testing moved from MML to testing internally at Children’s.
2/2/2012: Gliadin Ab IgG reference range updated.
5/15/2013: Gliadin IgG and IgA changed to Deamidated Gliadin IgG and IgA.