Lab Dept:


Test Name:


General Information

Lab Order Codes:



Anti Hepatitis C; Hepatitis C Antibody; HCVAB, Hepatitis C Viral Antibody; HCV Ab

CPT Codes:

86803 – Hepatitis C antibody 87522 – Hepatitis C, quantification (if appropriate)

Test Includes:

If Hepatitis C Virus (HCV) antibody screen by chemiluminescence immunoassay is reactive, then the presence of antibodies is confirmed at an additional charge.


Test Indications:

Screening for past (resolved) or chronic hepatitis C with automatic reflex to antibody confirmation test.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: HCPCR)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 2 days, test performed daily

Special Instructions:



Specimen Type:



Red top tube

Draw Volume:

6 mL (Minimum: 5 mL) blood

Processed Volume:

2 mL (Minimum: 1.7 mL) serum


Routine venipuncture


Special Processing:

Lab Staff: Centrifuge specimen within 1 hour of collection, remove serum aliquot into screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:


Sample Rejection:

Gross lipemia; gross hemolysis; grossly icteric; warm specimens; mislabeled or unlabeled specimens


Reference Range:

Negative (reported as negative or positive)

Note: Positive results will be confirmed by PCR at an additional charge.

Critical Values:



Serologic tests are not useful for detection or diagnosis of acute hepatitis C Virus (HCV) (< 2 months from exposure). Immunocompromised patients may not develop detectable anit-hepatitis C virus (anti-HCV) until 6 months after infection. Testing for Hepatitis C Virus RNA Detection and Quantification by Real-Time Reverse Transcription-PCR, Serum is recommended for detection of HCV infection in such patients.

Serologic test results are not useful for differentiating between past (resolved) and chronic hepatitis C. Such differentiation is best determined by detection of HCV RNA Detection and Quantification by Real-Time Reverse Transcription-PCR, Serum.

Infants born to HCV-infected mothers may have false-reactive HCV antibody test results due to transplacental passage of maternal HCV IgG antibodies. HCV antibody testing is not recommended until at least 18 months of age in these infants.

Performance characteristics have not been established for the following types of serum specimen:
Individuals of <10 years of age
● Grossly icteric (total bilirubin level of >20 mg/dL)
● Grossly lipemic (triolein level of >3,000 mg/dL)
● Grossly hemolyzed (hemoglobin level of >500 mg/dL)
● Presence of particulate matter
● Cadaveric specimens


Chemiluminescence immunoassay (CIA)
RT-PCR for confirmation, if appropriate


Mayo Medical Laboratories August 2013


4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratory. Test now includes an automatic reflexive confirmatory test done by RIBA when the EIA is positive.
3/9/2005: Test method previously listed as EIA. EDTA removed as an acceptable specimen anticoagulant.
11/12/2007: Name update to remove confirmation method from title.
2/25/2011: Confirmation by RIBA no longer available. Confirmation now by RT-PCR.
4/26/2011: Confirmation by RIBA once again available and will be the reflex.
3/16/2012: Confirmation by PCR as RIBA method is down.