Lab Dept:

Transfusion Services

Test Name:

PLATELET TRANSFUSION

General Information

Lab Order Codes:

TPLT

Special charges:

HLA Matched-MHLA, AHLA PLA1 Negative – PLAT, APLA1
Platelet Crossmatching PLTX – CPLT

Synonyms:

Leukocyte Reduced Platelets; Random Platelets; Platelet Rich Plasma; Platelet concentrate; Leukocyte Reduced Platelet Pheresis; Single-Donor Platelets; SDP Platelet pheresis; Apheresis Platelets; LRPH

CPT Codes:

P9035 – Platelet pheresis, Leukocyte reduced
86806 – Lymphocytotoxicity assay, visual crossmatch, without titration
86813 – HLA Matched
86945 – Irradiation
86985 – Volume Reduction
86965 – Pooling
86903 – Platelet Antigen typing
86999 – Washing
86022 – Platelet Crossmatch

Test Includes:

Leukocyte Reduced Platelet Pheresis consists of platelets suspended in 200-300 mL of plasma collected by cytapheresis. Each unit contains at least 3 x 1011 platelets, and ≤5.0 x 106 leukocytes. One pheresis unit equals 5-6 random unit platelet concentrate.

Logistics

Test Indications:

Refer to Guidelines for the Transfusion of Blood Components.

Platelet Crossmatching or HLA-Matched Platelets may be useful for patients receiving repeated platelet transfusions who have become refractile, and for patients who repeatedly develop febrile non-hemolytic transfusion reactions after platelet transfusions.

Volume Reduced Platelets are indicated in the event that ABO - incompatible platelets must be transfused due to availability or in patients with severe fluid restrictions. Refer to Blood Component Compatibility Chart

Lab Testing Sections:

Transfusion Services

Phone Numbers:

MIN Lab: 612-813-6824

STP Lab: 651-220-6558

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 2 hours

Standard Dose/Volume:

<10 kg: 10 – 15 mL/kg up to one unit or 50 mL maximum

10 – 15 kg: 1/3 pheresis unit

15 – 25 kg: 1/2 pheresis unit

>25 kg: 1 pheresis unit

Rate of Infusion:

10 minutes/unit, <4 hours

Administration:

Must be administered through a blood component administration filter. Volumes ≤60 mL may be issued by the Transfusion Service in prefiltered syringes.

Crossmatch:

Platelets must be ABO compatible. Crossmatch is not required.

Irradiation:

Yes for specific patient populations.

Order Instructions:

Indicate number of units or volume in mL’s; time and dose needed; and indication for transfusion.

Specify on the Transfusion Request Order Form special needs: Irradiate, Volume Reduce, Washed, Platelet Crossmatch, PLA1 negative, HLA matched.

Expiration:

Store at 20 – 24ºC with continuous agitation for 5 days. Pooled or aliquoted platelet transfusions must begin within 4 hours of preparation.

Do Not refrigerate.

Specimen

Specimen Type:

Refer to ABO/Rh if patient requires testing

Patient Preparation:

The patient must have a Medical Records band for checking against the component Unit Tag and the Transfusion Request Order Form prior to administration.

Interpretive

Methodology:

Platelets contain stable coagulation factors and labile factors V and VIII. Refer to Blood Component General Information for greater detail.

Contraindications:

Refer to Guidelines for Transfusion of Blood Components.

References:

Circular of Information of the Use of human Blood and Blood Components (2002) AABB, American Red Cross, America’s Blood Centers

Updates:

11/08/2004: Note change to platelet dosage for patients <20 kg.
8/07/2007: Update for platelet dosage levels.
2/12/2013: CPT update
3/12/2013: Removed references to pooled platelets